Recombinant Mouse Ephrin-B2 Fc Chimera Biotinylated Protein

R&D Systems, part of Bio-Techne | Catalog # BT496

R&D Systems, part of Bio-Techne
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BT496
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Key Product Details

Source

NS0

Accession #

AAA82934

Structure / Form

Disulfide-linked homodimer

Conjugate

Biotin

Applications

Binding Activity

View all Ephrin-B2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Ephrin-B2 protein
Mouse Ephrin-B2
(Arg27 - Ala227)
Accession # AAA82934		 DIEGRMD Human IgG1
(Pro100 - Lys330)		 6-His tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg27

Predicted Molecular Mass

49.6 kDa (monomer)

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized recombinant mouse EphB2 Fc Chimera at 2 µg/mL (100 µL/well) can bind Biotinylated Recombinant Mouse Ephrin-B2 Fc Chimera with a linear range of 0.039-2.5 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

BT496
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-B2

Ephrin‑B2, also known as Htk‑L, ELF‑2, LERK‑5, and NLERK‑1, is a 40 kDa member of the Ephrin‑B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains of Ephrin‑B ligands are structurally related to GPI‑anchored Ephrin‑A ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin‑B2 preferentially interacts with receptors in the EphB family (1, 2). Mature mouse Ephrin‑B2 consists of a 204 amino acid (aa) extracelluar domain (ECD), a 21 aa transmembrane segment, and an 83 aa cytoplasmic domain [Cerretti 1197, Bergemann 4921, Bennett 1866]. Within the ECD, mouse Ephrin‑B2 shares 97% and 98% aa sequence identity with human and rat Ephrin‑B2, respectively. Ephrin‑B2 is expressed presynaptically on neurons (6, 7). It promotes presynaptic development, EphB2 shedding, axonal growth cone collapse, and neurite repulsion, and also regulates inflammatory and neuropathic pain (6 ‑ 8). Ephrin‑B2 is expressed by vascular mural cells and arterial vascular and lymphatic endothelium (9, 10). It exerts proliferative and migratory effects on these cells during angiogenesis and lymphangiogenesis in part by regulating the signaling activity of VEGF R2 and VEGF R3 (8 ‑ 11). Ephrin‑B2 plays a role in the immune response by mediating monocyte extravasation and T cell costimulation (12, 13). It is upregulated in invasive cancers and promotes tumor cell migration, invasion, and tumor angiogenesis (14  ‑ 16). It functions as a cellular entry receptor for Hendra and Nipah viruses (17). Ephrin‑B2 is also important for the separation of the urinary and intestinal tracts during development (18).

References

  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Cerretti, D.P. et al. (1995) Mol. Immunol. 32:1197.
  4. Bergemann, A.D. et al. (1995) Mol. Cell. Biol. 15:4921.
  5. Bennett, B.D. et al. (1995) Proc. Natl. Acad. Sci. 92:1866.
  6. McClelland, A.C. et al. (2009) Proc. Natl. Acad. Sci. 106:20487.
  7. Zhao, J. et al. (2010) Mol. Pain 6:77.
  8. Lin, K.-T. et al. (2008) J. Biol. Chem. 283:28969.
  9. Foo, S.S. et al. (2006) Cell 124:161.
  10. Wang, Y. et al. (2010) Nature 465:483.
  11. Sawamiphak, S. et al. (2010) Nature 465:487.
  12. Pfaff, D. et al. (2008) J. Cell Sci. 121:3842.
  13. Yu, G. et al. (2003) J. Immunol. 171:106.
  14. Meyer, S. et al. (2005) Int. J. Oncol. 27:1197.
  15. Nakada, M. et al. (2010) Int. J. Cancer 126:1155.
  16. Liu, W. et al. (2004) Br. J. Cancer 90:1620.
  17. Bonaparte, M.I. et al. (2005) Proc. Natl. Acad. Sci. 102:10652.
  18. Dravis, C. et al. (2004) Dev. Biol. 271:272.

Alternate Names

EFNB2, ELF-2, EphrinB2, Htk-L, LERK-5, NLERK-1

Entrez Gene IDs

1948 (Human); 13642 (Mouse); 30219 (Zebrafish)

Gene Symbol

EFNB2

UniProt

AAA82934

Additional Ephrin-B2 Products

Product Documents for Recombinant Mouse Ephrin-B2 Fc Chimera Biotinylated Protein

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