Recombinant Mouse FGFR1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10135-FR

R&D Systems, part of Bio-Techne
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10135-FR-050
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Key Product Details

Source

NS0

Accession #

P16092-6

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all FGFR1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse FGFR1 protein
Mouse FGF R1
(Arg22-Ile376)
Accession # P16092-6
 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg22

Predicted Molecular Mass

66 kDa

SDS-PAGE

95-115 kDa, under reducing conditions

Activity

Measured by its ability to inhibit FGF acidic-dependent proliferation of NR6R-3T3 mouse fibroblast cells.
The ED50 for this effect is 15-90 ng/mL

Scientific Data Images for Recombinant Mouse FGFR1 Fc Chimera Protein, CF

Recombinant Mouse FGFR1 Fc Chimera Protein Inhibition Activity

Recombinant Mouse FGFR1 Fc Chimera Protein Inhibition Activity

Recombinant Mouse FGF R1 Fc Chimera (Catalog # 10135-FR) inhibits Recombinant Mouse FGF acidic (Catalog # 4686-FA) induced proliferation of NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is 15-90 ng/mL.
Recombinant Mouse FGFR1 Fc Chimera Protein SDS-PAGE

Recombinant Mouse FGFR1 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse FGF R1 Fc Chimer (Catalog # 10135-FR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 95-115 kDa and 190-230 kDa, respectively.

Formulation, Preparation and Storage

10135-FR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR1

Fibroblast growth factor receptor 1 (FGF R1) is a member of a tyrosine kinase family that acts as cell-surface receptors for fibroblast growth factors (FGFs). The FGF R family consists of four members that differ in their ligand affinities and play essential roles in the regulation of embryonic development, cell proliferation, differentiation, and migration (1, 2). Mature mouse FGF R1 consists of a 355 amino acid (aa) extracellular domain (ECD) composed of three immunoglobulin-like domains, a single transmembrane domain and a cytoplasmic tyrosine kinase domain (1). The ECD of mouse FGF R1 shares 98% and 99% aa identity with the human and rat ECD, respectively. FGF R1 binds both acidic and basic fibroblast growth factors and is involved in limb induction (2, 3). Alternative splice variants of this gene have been reported. This product is the longest variant, isoform 1 (1, 4). FGF Rs play essential roles in the regulation of embryonic development, cell proliferation, differentiation and migration (5). Upon FGF and heparan sulfate binding, the FGF Rs form homodimers and the kinase domains are activated (6, 7). Alterations in the binding regulates FGF bioactivity (8).

References

  1. Beer, H.-D. et al. (2000) J. Biol. Chem. 275:16091.
  2. Ornitz, D.M. et al. (1992) Mol. Cell. Bio. 12:240.
  3. Cuiling, Li. et al. (2005) Dev. 132:4755.
  4. Kouhara, H. et al. (1991) Biochem. Biophys. Res. Commun. 176:31.
  5. Turner, N and Grose, R. (2010) Nat. Rev. Cancer 10:116.
  6. Pantoliano, M.W. et al. (1994) Biochemistry 33:10229.
  7. Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
  8. Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.

Long Name

Fibroblast Growth Factor Receptor 1

Alternate Names

CD331, FGF R1, Flt-2

Entrez Gene IDs

2260 (Human); 14182 (Mouse)

Gene Symbol

FGFR1

UniProt

P16092-6

Additional FGFR1 Products

Product Documents for Recombinant Mouse FGFR1 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse FGFR1 Fc Chimera Protein, CF

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