Recombinant Mouse FGFR2 alpha (IIIb) Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5496-MF

R&D Systems, part of Bio-Techne
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5496-MF-050
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Key Product Details

Source

NS0

Accession #

ABL89196

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all FGFR2 alpha Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse FGF R2 alpha protein
Mouse FGF R2a (IIIb)
(Arg22 - Glu378) (Asn314His)
Accession # ABL89196		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg22, Ser24, and Leu27

Predicted Molecular Mass

66.2 kDa (monomer)

SDS-PAGE

105-120 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

200 ng/mL of biotinylated rmFGF1 is mixed with serially diluted rmFGF R2 alpha(IIIb)/Fc. Following incubation, the FGF R2 alpha(IIIb)/Fc-FGF1/biotin complex is captured on an EvenCoatTM Streptavidin Microplate (Catalog # CP003). Bound FGF R2 alpha(IIIb)/Fc is measured using HRP labeled Gt x Human IgG Fc. The concentration of rmFGF R2 alpha(IIIb)/Fc Chimera that produces 50% of the optimal binding was found to be approximately 150-600 ng/mL.

Formulation, Preparation and Storage

5496-MF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. 
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR2 alpha

Fibroblast growth factor receptor 2 alpha (FGF R2 alpha) is a 140 kDa type I transmembrane receptor tyrosine kinase that mediates the heparan sulfate proteoglycan-dependent biological activity of several FGFs. It is a member of the FGF R family of proteins whose extracellular domains (ECD) contain three immunoglobulin (Ig)-like domains with a series of acidic residues between the first and second Ig-like domains (1, 2). Multiple forms of FGF R2 are generated by alternative splicing (1, 2). Alpha isoforms contain all three Ig-like domains, while beta forms lack the first Ig-like domain (3, 4). The C-terminal portion of the third Ig-like domain can be encoded by either of two mutually exclusive exons, giving rise to the IIIb/KGFR and IIIc/BEK isoforms with distinct ligand selectivity (3, 4). This recombinant protein corresponds to the ECD from Accession # ABL89196. It shares 94% amino acid sequence identity with the ECD of human FGF R2 alpha (IIIb). FGF R2 alpha (IIIb) signals in response to FGF-1, -3, -7, -10, and -22, leading to receptor dimerization and autophosphorylation of its tyrosine kinase domain (1, 2, 5, 6). In contrast, the IIIc isoform preferentially responds to FGF-1, -2, -4, -5, -6, -8, -9, -16, -17, -18, and -20 (5, 6). FGF R2 alpha (IIIb) is widely expressed on epithelial cells, while its ligands are typically expressed by neighboring mesenchymal cells (2, 7). Its involvement in epithelial-mesenchymal interactions plays an important role in the morphogenesis of many tissues. Knockout mice exhibit widespread developmental defects due to an unresponsiveness to normal mitogenic stimulation (8 - 11). Mutations in FGF R2 alpha (IIIb) as well as altered splicing patterns (to isoform IIIc) are associated with a variety of skin and skeletal disorders and the progression of many epithelial cancers (12 - 14).

References

  1. Wiedlocha, A. and V. Sorensen (2004) Curr. Topics Microbiol. Immunol. 286:45.
  2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  3. Miki, T. et al. (1991) Science 251:72.
  4. Mansukhani, A. et al. (1992) Proc. Natl. Acad. Sci. 89:3305.
  5. Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
  6. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
  7. Finch, P.W. et al. (1995) Dev. Dyn. 203:223.
  8. Petiot, A. et al. (2003) Development 130:5493.
  9. Marguerie, A. et al. (2006) Cardiovasc. Res. 71:50.
  10. Revest, J.-M. et al. (2001) Dev. Biol. 231:47.
  11. Petiot, A. et al. (2005) Development 132:2441.
  12. Katoh, M. (2009) J. Invest. Dermatol. 129:1861.
  13. Su, N. et al. (2008) Front. Biosci. 13:2842.
  14. Acevedo, V.D. et al. (2009) Cell Cycle 8:580.

Long Name

Fibroblast Growth Factor Receptor 2 alpha

Alternate Names

FGF R2a

Entrez Gene IDs

2263 (Human); 14183 (Mouse)

Gene Symbol

FGFR2

UniProt

ABL89196

Additional FGFR2 alpha Products

Product Documents for Recombinant Mouse FGFR2 alpha (IIIb) Fc Chimera Protein, CF

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