Recombinant Mouse FLRT1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10955-FL

R&D Systems, part of Bio-Techne
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10955-FL-050
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Key Product Details

Source

NS0

Accession #

NP_958813.1

Conjugate

Unconjugated

Applications

Bioactivity

View all FLRT1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse FLRT1 protein
Thr52-Pro552

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr52

Predicted Molecular Mass

60 kDa

SDS-PAGE

65-83 kDa, under reducing conditions.

Activity

Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells.
The ED50 for this effect is 0.50-4.00 μg/mL.

Scientific Data Images for Recombinant Mouse FLRT1 His-tag Protein, CF

Recombinant Mouse FLRT1 His-tag Protein Bioactivity.

Recombinant Mouse FLRT1 His-tag Protein (Catalog # 10955-FL) supports the adhesion of Neuro 2A mouse neuroblastoma cells. The ED50 for this effect is 0.500-4.00 µg/mL.

Recombinant Mouse FLRT1 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Mouse FLRT1 His-tag Protein (Catalog # 10955-FL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-83 kDa.

Formulation, Preparation and Storage

10955-FL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FLRT1

Fibronectin-like domain-containing leucine-rich transmembrane protein 1 (FLRT1) is one of three FLRT leucine-rich repeat transmembrane glycoproteins involved in the regulation of FGF signaling. FLRTs are characterized by an extracellular domain (ECD) with fibronectin and leucine rich repeat (LRR) motifs involved in cell adhesion functions (1). Mature mouse FLRT1 consists of an ECD with 10 N-terminal leucine-rich repeats and a juxtamembrane fibronectin type III domain, a single transmembrane domain and a short cytoplasmic domain lacking defined signaling motifs (1). The ECD of mouse FLRT1 shares 97% amino acid (aa) sequence identity with the ECD of human FLRT1. Both during development and in adulthood, FLRT1 is expressed in distinct areas of the brain and other tissues, including the kidney, and Its expression is distinct from that of FLRT2 and FLRT3 (1, 2). In mouse, FLRT1 is expressed at brain compartmental boundaries in embryos (2). The fibronectin domain of all three FLRTs can bind FGF receptors and this binding is thought to regulate FGF signaling during development (2, 3). The LRR domains are responsible for both the localization of FLRTs in areas of cell contact and homotypic cell-cell association (4). This may be through direct interactions with other FLRT molecules or, as has been shown for FLRT3, by regulating internalization of adhesion molecules such as cadherins (4, 5). Down-regulation of FLRT1 has been associated with prognosis of gastric cancer and has potential to be a therapeutic target for tumor treatment (6).

References

  1. Lacy, S.E. et al. (1999) Genomics 62:417.
  2. Haines, B.P. et al. (2006) Dev. Biol. 297:14.
  3. Bottcher, R.T. et al. (2004) Nat. Cell Biol. 6:38.
  4. Karaulanov, E.E. et al. (2006) EMBO Rep. 7:283.
  5. Ogata, S. et al. (2007) Genes Dev. 21:1817.
  6. Liang, Y. et al. (2019) Pathol. Res. Pract. 215:152570.

Long Name

Fibronectin Leucine Rich Transmembrane Protein 1

Alternate Names

SPG68

Entrez Gene IDs

23769 (Human); 396184 (Mouse)

Gene Symbol

FLRT1

UniProt

NP_958813.1

Additional FLRT1 Products

Product Documents for Recombinant Mouse FLRT1 His-tag Protein, CF

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Product Specific Notices for Recombinant Mouse FLRT1 His-tag Protein, CF

For research use only

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