Recombinant Mouse Follistatin-like 5/FSTL5 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9065-FN

R&D Systems, part of Bio-Techne
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9065-FN-100
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Key Product Details

Source

CHO

Accession #

Q8BFR2

Conjugate

Unconjugated

Applications

Bioactivity

View all Follistatin-like 5/FSTL5 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Follistatin-like 5/FSTL5 protein
Arg21-Ala847, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg21

Predicted Molecular Mass

94 kDa

SDS-PAGE

85-98 kDa, reducing conditions

Activity

Measured by its ability to inhibit Hep3B human hepatocellular carcinoma cell survival.
The ED50 for this effect is 2-10 μg/mL.

Scientific Data Images for Recombinant Mouse Follistatin-like 5/FSTL5 Protein, CF

Recombinant Mouse Follistatin-like 5/FSTL5 Protein Bioactivity

Recombinant Mouse Follistatin-like 5/FSTL5 Protein Bioactivity

Recombinant Mouse Follistatin-like 5/FSTL5 (Catalog # 9065-FN) inhibits Hep3B human hepatocellular carcinoma cell survival. The ED50 for this effect is typicallly 2-10 μg/mL.

Formulation, Preparation and Storage

9065-FN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Follistatin-like 5/FSTL5

Follistatin-like 5 (FSTL5) is a secreted 94 kDa (predicted) member of the SPARC protein family (1). In its N-terminal half, FSTL5 contains a Kazal-like domain, two EF-hand domains, and two Ig-like domains. Mature mouse FSTL5 shares 91% and 97% amino acid sequence identity with human and rat FSTL5, respectively. FSTL5 is expressed in the cartilage and tendon of developing digits and the shafts of long bones (2). In the brain it is expressed in the olfactory bulb, CA3 area of the hippocampus, and granule cell layer of the cerebellum (3). FSTL5 inhibits the proliferation and promotes the apoptosis of hepatocellular carcinoma cells (4). Its expression in medulloblastoma and downregulation in hepatocellular carcinoma are associated with poor prognosis (4, 5).

References

  1. Bradshaw, A.D. (2012) Int. J. Biochem. Cell Biol. 44:480.
  2. Lorda-Diez, C.I. et al. (2013) PLoS One 8:e60423.
  3. Masuda, T. et al. (2014) Congenit. Anom. (Kyoto) 54:63.
  4. Zhang, D. et al. (2015) Int. J. Clin. Exp. Pathol. 8:3386.
  5. Remke, M. et al. (2011) J. Clin. Oncol. 29:3852.

Alternate Names

Follistatin like 5, FSL5, FSTL5

Entrez Gene IDs

56884 (Human); 213262 (Mouse); 365823 (Rat)

Gene Symbol

FSTL5

UniProt

Q8BFR2

Additional Follistatin-like 5/FSTL5 Products

Product Documents for Recombinant Mouse Follistatin-like 5/FSTL5 Protein, CF

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Product Specific Notices for Recombinant Mouse Follistatin-like 5/FSTL5 Protein, CF

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