Recombinant Mouse Frizzled-5 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9060-FZ

R&D Systems, part of Bio-Techne
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9060-FZ-050
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Key Product Details

Source

NS0

Accession #

Q9EQD0

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Frizzled-5 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Frizzled-5 protein
Mouse Frizzled-5
(Ala27-Pro167)
Accession # Q9EQD0		 IEGRMD Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala27

Predicted Molecular Mass

43 kDa

SDS-PAGE

54-64 kDa, reducing conditions

Activity

Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.
The ED50 for this effect is 10-60 ng/ml in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN).

Scientific Data Images for Recombinant Mouse Frizzled-5 Fc Chimera Protein, CF

Recombinant Mouse Frizzled-5 Fc Chimera Protein Bioactivity

Recombinant Mouse Frizzled-5 Fc Chimera Protein Bioactivity

Recombinant Mouse Frizzled-5 FC Chimera (Catalog # 9060-FZ) inhibits Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induced alkaline phosphatase production in the MC3T3-E1 mouse preosteoblast cell line. The ED50 for this effect is 0.01-0.06 µg/ml.

Formulation, Preparation and Storage

9060-FZ
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-5

Frizzled-5 is an approximately 62 kDa 7-TM glycoprotein that is one of at least ten Frizzled family Wnt receptors (1, 2). Wnt engagement of Frizzled, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co‑receptors, stabilizes beta-catenin and promotes gene transcription that is important in development and tissue maintenance (1). Frizzleds can also signal through non-canonical pathways independently of LRPs (1). Mature mouse Frizzled-5 consists of a 212 amino acid (aa) extracellular domain (ECD), a 283 aa 7-TM region, and a 64 aa cytoplasmic domain with a PDZ binding motif (2, 3). The ECD includes a cysteine-rich region (CRD) that binds Wnts and is highly conserved among Frizzled proteins (1, 2, 4). Within the N-terminal ECD, human Frizzled-5 shares 95% aa sequence identity with mouse and rat Frizzled-5. Frizzled-5 is expressed in embryonic tissues (telencephalon, pituitary, thalamus, hypothalamus, eye, liver, spleen, lung, and kidney), in the adult retina, colon, and pancreatic islets, some cancer cell lines, human embryonic stem cells, and in some monocyte and lymphocyte populations (2-8). Frizzled-5 functions as a receptor for Wnt-5a, Wnt-9b, Wnt-10b, Wnt-2b, and Wnt-7a (3, 5, 9, 10). It plays a role in the maintenance of yolk sac and placental vasculature (ishikawa) and in the regression of vitreous vasculature during eye development (5, 6, 11). It mediates the synaptogenic effect of Wnt-7a, contributes to the development of neuronal polarity, and is required for neuronal survival in the thalamus (3, 12, 13). In macrophages and monocytes, Frizzled-5 signaling induces the production of inflammatory cytokines (7, 8).

References

  1. Schulte, G. (2015) Eur. J. Pharmacol. 763:191.
  2. Saitoh T. et al. (2001) Int. J. Oncol. 19:105.
  3. Liu, C. et al. (2008) J. Neurosci. 28:5641.
  4. Katoh, Y. and M. Katoh (2007) Int. J. Oncol. 30:751.
  5. Ishikawa, T. et al. (2001) Development 128:25.
  6. Burns, C.J. et al. (2008) Dev. Dyn. 237:1614.
  7. Periera, C. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:504.
  8. Blumenthal, A. et al. (2006) Blood 108:965.
  9. He, X. et al. (1997) Science 275:1652.
  10. Caricasole, A. et al. (2003) J. Biol. Chem 278:37024.
  11. Zhang, J. et al. (2008) Invest. Ophthalmol. Vis. Sci. 49:5561.
  12. Sahores, M. et al. (2010) Development 137:2215.
  13. Slater, P.G. et al. (2013) PLoS One 8:e78892.

Alternate Names

Frizzled5, Frz-5, FZ-5, FZD5

Entrez Gene IDs

7855 (Human); 14367 (Mouse)

Gene Symbol

FZD5

UniProt

Q9EQD0

Additional Frizzled-5 Products

Product Documents for Recombinant Mouse Frizzled-5 Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse Frizzled-5 Fc Chimera Protein, CF

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