Recombinant Mouse G-CSF Protein
R&D Systems, part of Bio-Techne | Catalog # 414-CS
Key Product Details
Product Specifications
Source
E. coli-derived mouse G-CSF protein
Val31-Ala208, with an N-terminal Met
Val31-Ala208, with an N-terminal Met
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
19 kDa
Activity
Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. Shirafuji, N.et al. (1989) Exp. Hematol. 17:116.
The ED50 for this effect is 10-60 pg/mL.
The ED50 for this effect is 10-60 pg/mL.
Reviewed Applications
Read 3 reviews rated 4.3 using 414-CS in the following applications:
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 414-CS
| Formulation | Lyophilized from a 0.2 μm filtered solution in Citric Acid and Tween® 20 with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile deionized water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 414-CS/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Citric Acid and Tween® 20 with Trehalose. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile deionized water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: G-CSF
The murine G-CSF cDNA encodes a 208 amino acid (aa) residue precursor protein containing a 30 aa residue signal peptide that is proteolytically cleaved to generate the 178 aa residue mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.
In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant.
Long Name
Granulocyte Colony Stimulating Factor
Alternate Names
C17orf33, CSF3, CSF3OS, Filgrastim, GCSF, Lenograstim, Pluripoietin
Gene Symbol
CSF3
UniProt
Additional G-CSF Products
Product Documents for Recombinant Mouse G-CSF Protein
Product Specific Notices for Recombinant Mouse G-CSF Protein
For research use only
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