Recombinant Mouse Gas6 (Full Length) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8310-GS

R&D Systems, part of Bio-Techne
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8310-GS-050
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Key Product Details

Source

NS0

Accession #

CAA42507

Conjugate

Unconjugated

Applications

Binding Activity

View all Gas6 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Gas6 protein
Ala46-Pro673, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala46 & Tyr47

Predicted Molecular Mass

71 kDa

SDS-PAGE

78-94 kDa, reducing conditions

Activity

Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. Mishra, A. et al. (2012) Mol. Cancer Res. 10:703.
The ED50 for this effect is 20-100 ng/mL.

Reviewed Applications

Read 1 review rated 4 using 8310-GS in the following applications:

Scientific Data Images for Recombinant Mouse Gas6 (Full Length) Protein, CF

Recombinant Mouse Gas6 (Full Length) Protein Bioactivity

Recombinant Mouse Gas6 (Full Length) Protein Bioactivity

Recombinant Mouse Gas6 (Catalog # 8310-GS) down regulates the expression of Axl in DU145 human prostate carcinoma cells. The ED50 for this effect is 20-100 ng/mL.
Recombinant Mouse Gas6 (Full Length) Protein SDS-PAGE

Recombinant Mouse Gas6 (Full Length) Protein SDS-PAGE

1 μg/lane of Recombinant Mouse Gas6 (Full Length, Catalog # 8310-GS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing R bands at 85.4 and 69.2 kDa and NR band at 64.4 kDa.

Formulation, Preparation and Storage

8310-GS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Gas6

Gas6 (Growth Arrest Specific 6) is a secreted 75 kDa multimodular protein that is up-regulated by a wide variety of cell types in response to growth arrest. It is expressed by endothelial cells, fibroblasts, neurons, smooth muscle cells, and platelets, and it plays a role in vascular, thrombotic, atherosclerotic, inflammatory, autoimmune, renal, and cancer pathologies (1, 2). Both Gas6 and the related Protein S contain an extensively gamma-carboxylated N-terminal Gla domain, four EGF-like repeats, and two C-terminal Laminin G-like domains. And like Protein S, Gas6 is dependent upon vitamin K for activity. Within the Gla, EGF-like, and Laminin G-like domains, mouse Gas6 shares 83%, 94%, and 41% aa sequence identity with the equivalent regions in human Gas6, rat Gas6, and mouse Protein S, respectively. Gas6 binds to and induces signaling through the receptor tyrosine kinase TAM subfamily, which includes Axl, Dtk/Tyro3, and Mer (3). Shed soluble forms of Axl and Mer retain the ability to bind Gas6 and function as decoy receptors (4, 5). Gas6 participates in tissue homeostasis by protecting cells from stress-induced apoptosis and promoting apoptotic cell phagocytosis. The affinity of the gamma-carboxylated Gla domain for phosphatidylserine contributes to the role of Gas6 in phagocytosis as well as the cellular entry of select viruses (6, 7). Gas6 can function as a pro-inflammatory molecule by promoting platelet activation (8, 9) and can also inhibit inflammatory cytokine production from monocytes, macrophages, and microglia (10). In addition, Gas6 induces the proliferation of cardiac fibroblasts, Schwann cells, vascular smooth muscle cells, and the differentiation of NK cell precursors (11-14). It also inhibits VEGF-induced angiogenesis (15) and can have either positive or negative effects on tumor cell proliferation and invasion (16, 17).

References

  1. Laurance, S. et al. (2012) Adv. Nutr. 3:196.
  2. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
  3. Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
  4. Sather, S. et al. (2007) Blood 109:1026.
  5. Ekman, C. et al. (2010) J. Thromb. Haemost. 8:838.
  6. Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790.
  7. Morizono, K. et al. (2011) Cell Host Microbe 9:286.
  8. Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733.
  9. Robins, R.S. et al. (2012) Blood 121:692.
  10. Alciato, F. et al. (2010) J. Leukoc. Biol. 87:869.
  11. Stenhoff, J. et al. (2004) Biochem. Biophys. Res. Commun. 319:871.
  12. Li, R. et al. (1996) J. Neurosci. 16:2012.
  13. Nakano, T. et al. (1995) J. Biol. Chem. 270:5702.
  14. Caraux, A. et al. (2006) Nat. Immunol. 7:747.
  15. Gallicchio, M. et al. (2005) Blood 105:1970.
  16. Loges, S. et al. (2010) Blood 115:2264.
  17. Gustafsson, A. et al. (2009) PLoS ONE 4:e7575.

Long Name

Growth-arrest-specific Protein 6

Alternate Names

AXLLG

Entrez Gene IDs

2621 (Human); 14456 (Mouse)

Gene Symbol

GAS6

UniProt

CAA42507

Additional Gas6 Products

Product Documents for Recombinant Mouse Gas6 (Full Length) Protein, CF

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