Recombinant Mouse GDF-9 Protein

R&D Systems, part of Bio-Techne | Catalog # 739-G9

R&D Systems, part of Bio-Techne
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Carrier Free
739-G9-010/CF
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With Carrier
739-G9-010
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Key Product Details

Source

CHO

Accession #

AAA53035

Conjugate

Unconjugated

Applications

Bioactivity

View all GDF-9 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse GDF-9 protein
Gly307-Arg441

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly307

Predicted Molecular Mass

15.6 kDa

SDS-PAGE

20 kDa, reducing conditions

Activity

Measured by its ability to induce cell death using Mv1Lu mink lung epithelial cells.
The ED50 for this effect is 0.1-0.4 μg/mL.

Reviewed Applications

Read 1 review rated 4 using 739-G9 in the following applications:

Scientific Data Images for Recombinant Mouse GDF-9 Protein

Recombinant Mouse GDF-9 Protein Bioactivity

Recombinant Mouse GDF-9 Protein Bioactivity

Recombinant Mouse GDF-9 induces Mv1Lu mink lung epithelial cell death. The ED50 for this effect is 0.1-0.4 μg/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 739-G9
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl containing 0.1% bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 739-G9/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-9

Growth Differentiation Factor-9 (GDF-9) is an oocyte secreted paracrine factor in the TGF-beta superfamily (1, 2). It is synthesized as a prepropeptide and is subsequently processed by proteases into the mature protein (1, 2). Mature mouse GDF-9 has a predicted molecular weight of 15.6 kDa, and shares 90% and 95% amino acid sequence identity with mature human and rat GDF-9, respectively. It forms both non-covalent homodimers and heterodimers with BMP-15, which is coordinately expressed with GDF-9 in the oocyte. (3-5). GDF-9 signals through TGF-beta RI/ALK-5 and BMPR-II, while the GDF-9:BMP-15 heterodimer is believed to signal through BMPR-II, ALK-4, -5, -7, and BMPR-IB/ALK-6 (5-8). SMAD2 and SMAD3 are phosphorylated following activation of receptor complexes by GDF-9 (5, 6). GDF-9 functions as a paracrine factor in the development of primary follicles in the ovary (9, 10). It is critical for the growth of granulosa and theca cells and for the differentiation and maturation of the oocyte (11, 12). GDF-9 is thought to act synergistically with BMP-15 to control development of the oocyte-cumulus cell complex (5, 12-14). In mice, GDF-9:BMP-15 heterodimers have been shown to be more potent regulators of granulosa cell functions compared to GDF-9 homodimers (6). Studies on GDF-9 null mice have demonstrated arrested follicular development at the primary follicle stage (10). In humans, aberrant GDF-9 expression and activation is associated with a multitude of common human ovarian disorders including premature ovarian failure and polycystic ovary syndrome (15-17).

References

  1. McPherron, A.C. and S.J. Lee (1993) J. Biol. Chem. 268:3444.
  2. McGrath, S. A. et al. (1995) Mol. Endocrinol. 9:131.
  3. Laitinen, M. et al. (1998) Mech. Dev. 78:135.
  4. Liao, W.X. et al. (2003) J. Biol. Chem. 278:3713.
  5. Gilchrist, R.B. et al. (2008) Hum. Reprod. Update 14:159.
  6. Peng, J. et al. (2013) Proc. Natl. Acad. Sci. USA 110:E776.
  7. Vitt, U.A. et al. (2002) Biol. Reprod. 67:473.
  8. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:653.
  9. Elvin, J.A. et al. (1999) Mol. Endocrinol. 13:1035.
  10. Dong, J. et al. (1996) Nature 383:531.
  11. Hreinsson, J.G. et al. (2002) J. Clin. Endocrinol. Metab. 87:316.
  12. Su, Y.Q. et al. (2004) Dev. Biol. 276:64.
  13. Yan, C. et al. (2001) Mol. Endocrinol. 15:854.
  14. Mottershead, D.G. et al. (2012) Mol. Hum. Reprod. 18:121.
  15. Zhao, S.Y. et al. (2010) Fertil. Steril. 94:261.
  16. Wei, L.N. et al. (2011) Fertil. Steril. 96:464.
  17. Simpson, C.M. et al. (2014) J. Clin. Endocrinol. Metab. [Epub ahead of print].

Long Name

Growth Differentiation Factor 9

Alternate Names

GDF9

Entrez Gene IDs

2661 (Human); 14566 (Mouse)

Gene Symbol

GDF9

UniProt

AAA53035

Additional GDF-9 Products

Product Documents for Recombinant Mouse GDF-9 Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS 739-G9 (with carrier)
Download SDS 739-G9/CF (carrier free)

Product Specific Notices for Recombinant Mouse GDF-9 Protein

For research use only

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