Recombinant Mouse GFR alpha-like Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9844-GR

R&D Systems, part of Bio-Techne
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9844-GR-050
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Key Product Details

Source

CHO

Accession #

Q6SJE0-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all GFR alpha-like Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse GFR alpha-like protein
Mouse GFR alpha-like
(Gln20-Gly349)
Accession # Q6SJE0-1		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln20 inferred from enzymatic pyroglutamate treatment revealing Thr21

Predicted Molecular Mass

64 kDa

SDS-PAGE

66-85 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse GFR alpha‑like Fc Chimera is coated at 0.1 μg/mL, 100 μL/well, it binds to Biotinylated Recombinant Mouse GDF-15 with an ED50 of 0.3-1.8 ng/mL.

Scientific Data Images for Recombinant Mouse GFR alpha-like Fc Chimera Protein, CF

Recombinant Mouse GFR alpha-like Fc Chimera Protein Bioactivity

Recombinant Mouse GFR alpha-like Fc Chimera Protein Bioactivity

When Recombinant Mouse GFR alpha‑like Fc Chimera (Catalog # 9844-GR) is immobilized at 0.1 μg/mL, 100 μL/well, It binds to Biotinylated Recombinant Mouse GDF-15 with an ED50 of 0.3-1.8 ng/mL.

Formulation, Preparation and Storage

9844-GR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: GFR alpha-like

GFR alpha-like (GDNF receptor-alpha-like) is a 25 kDa dimer and distant member of the GDNFR family of proteins (1). It is expressed on both fetal and adult hindbrain neurons of the CNS (2), and would appear to function as an anti-apoptotic molecule during neuronal stress. GFR alpha-like is a funcation receptor of GDF15 and implicated in diet-based obesity and insulin resistance through c-Ret signaling (2 - 4). Mature mouse GFR alpha-like is a 374 amino acid (aa) type I transmembrane protein. It contains a 330 aa extracellular region that shows a Gas1 domain (aa 220‑316), and a 23 aa cytoplasmic tail. Over aa 19‑353, mouse GFR alpha-like shares 72% and 71% aa identity with human and rat GFR alpha–like, respectively.

References


  1. Li, Z. et al. (2005) J. Neurochem. 95:361.
  2. Yang, L. et al. (2017) Nature Medicine 23:1158.
  3. Mullican, S. et al. (2017) Nature medicine 23:1150.

  4. Emmerson, P. et al. (2017) Nature Medicine 23:1215.

Long Name

Glial Cell line-derived Neurotrophic Factor Receptor alpha-like

Alternate Names

GFR alpha like, GFRAL, GRAL

Entrez Gene IDs

389400 (Human); 404194 (Mouse); 501023 (Rat)

Gene Symbol

GFRAL

UniProt

Q6SJE0-1

Additional GFR alpha-like Products

Product Documents for Recombinant Mouse GFR alpha-like Fc Chimera Protein, CF

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