Recombinant Mouse Granzyme G Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1346-SE
Key Product Details
Source
Sf 21 (baculovirus)
Accession #
Structure / Form
Mature form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Granzyme G protein
Ile21-Leu248, with a C-terminal 10-His tag
Accession # P13366
Ile21-Leu248, with a C-terminal 10-His tag
Accession # P13366
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ile21
Predicted Molecular Mass
27 kDa
SDS-PAGE
35 kDa, reducing conditions
Activity
Measured by its ability to cleave a peptide substrate, Succinyl-Phe-Leu-Phe-ThioBenzyl ester (Suc-FLF-SBzl), in the presence of 5,5’-Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
The specific activity is >150 pmol/min/µg, as measured under the described conditions.
The specific activity is >150 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
1346-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Granzyme G
Granzyme G is a member of the Granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1). Together with Granzymes D, E and F, it is regulated through pregnancy and by IL-2 and IL-15 in granulated metrial gland cells (2). Human or rat counterpart of mouse Granzyme G has not been found. Like other Granzymes, mouse Granzyme G is not secreted as a zymogen but stored as a fully processed and activated enzyme in the cytoplasmic granules of CTL (3). It is synthesized as a 248 amino acid precursor with a 18 amino acid signal peptide and a 2 amino acid propeptide (3, 4). The mature protein (residues 21‑248) is expressed and purified.
References
- Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
- Allen, M.P. and M. Nilsen-Hamilton (1998) J. Immunol. 161:2772.
- Jenne, D.E. et al. (1989) Biochemistry 28:7953.
- Kwon, B.S. et al. (1988) J. Exp. Med. 168:1839.
Alternate Names
GrzG, GZMG
Gene Symbol
GZMG
UniProt
Additional Granzyme G Products
Product Documents for Recombinant Mouse Granzyme G Protein, CF
Product Specific Notices for Recombinant Mouse Granzyme G Protein, CF
For research use only
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