Recombinant Mouse Hepsin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9156-SE

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
9156-SE-010
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

O35453-2

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Hepsin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Hepsin protein
Gln44-Pro416 (Asp160Glu & Arg161Lys), with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln44 inferred from enzymatic pyroglutamate treatment revealing Ser45

Predicted Molecular Mass

42 kDa

SDS-PAGE

41-47 kDa, reducing conditions

Activity

Measured by its ability to cleave tert-butoxycarbonyl-Gln-Arg-Arg-7-amino-4-methylcoumarin (Boc-QRR-AMC).
The specific activity is >8,000 pmol/min/μg, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 4 using 9156-SE in the following applications:

Formulation, Preparation and Storage

9156-SE
Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Hepsin

Hepsin, also known as TMPRSS1, is an approximately 50 kDa Type II membrane protein with an extracellular serine protease domain. It is primarily expressed in liver as a disulfide linked heterodimer of heavy and light chains (1, 2). Hepsin cleaves and activates Coagulation Factors VII, IX, and XII (3, 4), the pro forms of HGF, MSP, and uPA (4-8), and Prostasin/PRSS8 (9). It also cleaves EGF R and prevents receptor binding of EGF [9]. Its enzymatic activity is inhibited by HAI-1 and HAI-2 (4, 5, 8). Hepsin is overexpressed in various human tumors including prostate and breast (6, 10), and it promotes tumor progression and metastasis (11). It disrupts attachment of epithelial cells to the basement membrane by inducing the breakdown of  desmosomal structures (6). Hepsin also binds to Protein X from hepatitis B virus and to pORF3 from hepatitis E virus (12, 13). Within the ECD, mouse Hepsin shares 89% and 98% amino acid (aa) sequence identity with human and rat Hepsin, respectively (2). An alternatively spliced isoform has a 20 aa deletion in the cytoplasmic and transmembrane regions (2).

References

  1. Tsuji, A. et al. (1991) J. Biol. Chem. 266:16948.
  2. Vu, T.-K.H. et al. (1997) J. Biol. Chem. 272:31315.
  3. Kazama, Y. et al. (1995) J. Biol. Chem. 270:66.
  4. Herter, S. et al. (2005) Biochem. J. 390:125.
  5. Kirchhofer, D. et al. (2005) FEBS Lett. 579:1945.
  6. Tervonen, T.A. et al. (2016) Oncogene 35:1832.
  7. Ganesan, R. et al. (2011) Mol. Cancer Res. 9:1175.
  8. Moran, P. et al. (2006) J. Biol. Chem. 281:30439.
  9. Chen, M. et al. (2010) Mol. Cell. Biochem. 337:259.
  10. Dhanasekaran, S.M. et al. (2001) Nature 412:822.
  11. Li, W. et al. (2009) Cancer Res. 69:8395.
  12. Zhang, M. et al. (2015) Oncotarget 7:7780.
  13. Wang, C. et al. (2014) Hepat. Mon. 14:e13902.

Alternate Names

HPN, TMPRSS1

Entrez Gene IDs

3249 (Human); 15451 (Mouse); 29135 (Rat)

Gene Symbol

HPN

UniProt

O35453-2

Additional Hepsin Products

Product Documents for Recombinant Mouse Hepsin Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse Hepsin Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.