Recombinant Mouse HGF Propeptide Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7058-HG

R&D Systems, part of Bio-Techne
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7058-HG-010
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Key Product Details

Source

Sf 21 (stably transfected)

Accession #

Q08048

Conjugate

Unconjugated

Applications

Bioactivity

View all HGF Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (stably transfected)-derived mouse HGF protein
Gln33-Leu728

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln33 predicted, sequencing might be blocked

Predicted Molecular Mass

79.3 kDa

SDS-PAGE

84 kDa, reducing conditions

Activity

>40 ng of Recombinant Mouse HGF Propeptide is cleaved to release active HGF when treated for 4 hours at 37 °C with 10 ng of kallikrein.

Formulation, Preparation and Storage

7058-HG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HGF

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1-7). Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive
disulfide‑linked HGF with a 60 kDa alpha and 30 kDa beta chain. Alternate splicing generates an additional HGF isoform with a 5 aa deletion in the first Kringle domain. Mouse Pro‑HGF shares 92%-93% aa sequence identity with bovine, canine, feline, and human Pro-HGF and 99% with rat Pro-HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (8, 9). HGF-dependent c-MET activation is implicated in the development of many human cancers (10). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (11, 12). HGF induces the up-regulation of Integrin  alpha2 beta1 in epithelial cells by a selective increase in alpha2 gene transcription (13). This Integrin serves as a Collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (13, 14). HGF can also alter epithelium morphology by the induction of Nectin‑1 alpha ectodomain shedding, an adhesion protein component of adherens junctions (15). In the thymus, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (16). HGF promotes the motility of cardiac stem cells in damaged myocardium (17).

References

  1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
  2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
  3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
  4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
  5. Nakamura, T. et al. (1989) Nature 342:440.
  6. Liu, Y. et al. (1993) Biochim. Biophys. Acta 1216:299.
  7. Lee, C.C. et al. (1993) Cell Adhes. Commun. 1:101.
  8. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
  9. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
  10. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  11. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
  12. Montesano, R. et al. (1991) Cell 67:901.
  13. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
  14. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
  15. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
  16. Mineo, R. et al. (1994) Endocrinology 145:4355.
  17. Urbanek, K. et al. (2005) Circ. Res. 97:663.

Long Name

Hepatocyte Growth Factor

Alternate Names

DFNB39, F-TCF, Hepatopoietin A, HGFB, HPTA, SF

Entrez Gene IDs

3082 (Human); 15234 (Mouse); 24446 (Rat); 403441 (Canine); 102133907 (Cynomolgus Monkey); 493705 (Feline)

Gene Symbol

HGF

UniProt

Q08048

Additional HGF Products

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Product Specific Notices for Recombinant Mouse HGF Propeptide Protein, CF

For research use only

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