Recombinant Mouse Hip Protein
R&D Systems, part of Bio-Techne | Catalog # 1568-HP
Key Product Details
Product Specifications
Source
Mouse Hip (Lys24 - Arg678 & Asp52 - Arg678) Accession # AAD31172 |
IEGRMDGGGSGGGSGGGS | 10-His tag |
N-terminus | C-terminus |
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The ED50 for this effect is 1.5-7.5 µg/mL in the presence of 5 µg/mL rmShh.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 1568-HP
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Carrier Free: 1568-HP/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Hip
Hedgehog signaling proteins act as mitogens, morphogens, or inducing factors in many different cell types during embryonic development. They aid in growth, patterning, and morphogenesis in both vertebrates and insects (1). Hip (Hedgehog-interacting protein) is a type I transmembrane protein identified for its ability to bind biologically active Sonic Hedgehog. It is comprised of 700 aa, and includes a hydrophobic signal sequence, two EGF-like domains near the C-terminus, and a 22 amino acid membrane-spanning region at the C-terminal end (2). Hip has only been identified in vertebrates and binds all three mammalian Hedgehogs: sonic (Shh), desert (Dhh), and Indian (Ihh). Like the Hedgehog receptor Patched, Hip is a transcriptional target of Hedgehog signaling (2). Unlike Patched, Hip’s ability to bind hedgehogs is not involved in transducing a signal intracellularly, rather it regulates the availability of Hedgehog ligand extracellularly (3). Transgenic mice overexpressing Hip in proliferating chondrocytes display skeletal defects similar to those observed in Ihh mutant mice. These results indicate that Hip is involved in attenuating Hedgehog signaling (2). The expression pattern of Hip correlates with its ability to interact with all three mammalian Hedgehogs. It is expressed in a variety of organs, adjacent with sites of hedgehog expression. For instance, Shh is expressed in the epithelium of the lung, and Hip is found in the underlying lung mesenchyme (2). In fact, Hip knock-out mice exhibit neonatal lethality with respiratory failure due to defective branching morphogenesis. This phenotype correlates with altered expression of Shh markers suggesting an increase in Shh signaling (3). Interestingly, other developmental mechanisms that rely on normal Shh signaling, such as dorsal-ventral patterning of the neural tube, development of the somites, and organ laterality appeared histologically normal in Hip-/- mice (3). Mouse and human Hip share 94% amino acid identity through the entire protein sequence (4). R&D Systems’ recombinant Hedgehog-interacting protein is a potent antagonist of Shh signaling in vitro.
References
- Ingham, P.W. and A.P. McMahon (2001) Genes & Dev 15:3059.
- Chuang, P-T. and A.P. McMahon (1999) Nature 397: 17.
- Chuang, P-T. et al. (2003) Genes & Dev. 17:342.
- Bak, M. et al. (2001) Cytogenet. Cell Genet. 92:300.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional Hip Products
Product Documents for Recombinant Mouse Hip Protein
Product Specific Notices for Recombinant Mouse Hip Protein
For research use only