Recombinant Mouse IFN-alpha 2/IFNA2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10149-IF

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
10149-IF-010
10149-IF-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

HEK293

Accession #

P01573

Conjugate

Unconjugated

Applications

Bioactivity

View all IFN-alpha 2/IFNA2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 2/IFNA2 protein
Cys24-Glu190

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys24

Predicted Molecular Mass

19.3 kDa

SDS-PAGE

18-22 kDa, under reducing conditions

Activity

Measured in an anti-viral assay using L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681.
The ED50 for this effect is 20-200 pg/mL.

Scientific Data Images for Recombinant Mouse IFN-alpha 2/IFNA2 Protein, CF

Recombinant Mouse IFN-alpha 2/IFNA2 Protein Bioactivity

Recombinant Mouse IFN-alpha 2/IFNA2 Protein Bioactivity

Recombinant Mouse IFN-alpha 2/IFNA2 (Catalog # 10149-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 20-200 pg/mL.
Recombinant Mouse IFN-alpha 2/IFNA2 Protein SDS-PAGE

Recombinant Mouse IFN-alpha 2/IFNA2 Protein SDS-PAGE

2 μg/lane of Recombinant Mouse IFN-alpha 2/IFNA2 (Catalog # 10149-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-22 kDa.

Formulation, Preparation and Storage

10149-IF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage

Background: IFN-alpha 2/IFNA2

Interferon-alpha 2 (IFN alpha-2) is one of 14 subtypes within the IFN-alpha class of Type I Interferons (1). The members of the IFN-alpha class, also known as alpha leukocyte interferons, encompass a group of distinct but closely related proteins which share approximately 80% amino acid (aa) sequence identity and have a similar globular structure composed of five alpha-helices (1, 3, 4). IFN-alpha class members signal through a common cell surface receptor complex composed of IFN-alpha R2 and IFN-alpha R1 subunits (3). As the first highly active IFN to be cloned and produced, IFN alpha-2 has become the prototypic IFN for academic and pharmaceutical research (2). The mature extracellular domain (ECD) of mouse IFN alpha-2 shares 60% and 83% aa sequence identity with human and rat, respectively. Murine IFN-alpha 2 can eliminate cardiac viral load and protect cardiomyocytes from injury in animals infected with coxsackievirus B3 (CVB3) (5). IFN alpha-2 derived mutants with reduced IFNR2 binding inhibited HIV replication and mutants with more IFNAR1 binding potentiated antiviral activity (6).

References

  1. Pestka, S. (2007) J Biol Chem. 282:20047.
  2. Paul, F. et al. (2015) Gene. 567(2):132.
  3. Oritani, K. et al. (2001). Cytokine & Growth Factor Reviews, 12:337.
  4. Pesch, V. et al. (2004). Journal of Virology, 78:8219.
  5. Wang, Y.X. et al. (2007) Am J Physiol Heart Circ Physiol. 293:H69.
  6. Schlaepfer, E. et al. (2019) Am Soc for Microbiology 4:e00637.

Long Name

Interferon alpha 2

Alternate Names

Ifa2, IFNA2, IFNA2a, IFNA2b, IFNA2c, IFNAA, IFNalpha 2

Entrez Gene IDs

3440 (Human); 15965 (Mouse); 298213 (Rat)

Gene Symbol

IFNA2

UniProt

P01573

Additional IFN-alpha 2/IFNA2 Products

Product Documents for Recombinant Mouse IFN-alpha 2/IFNA2 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse IFN-alpha 2/IFNA2 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.