Recombinant Mouse IFN-beta (Mammalian) Protein, CF

Name: Recombinant Mouse IFN-beta (Mammalian) Protein, CF
Brand: R&D Systems
SKU: 12410-1

R&D Systems, part of Bio-Techne | Catalog # 12410-1

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Citations (2)

Product Datasheet / COA / SDS

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Accession #

K00020

Conjugate

Unconjugated

Applications

Bioactivity

View all IFN-beta Proteins and Enzymes »

Product Specifications

Source

Gene was obtained from mouse DNA expressed in mammalian cells.
Accession # K00020

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

19.6 kDa

Activity

Interferon was titrated with the use of the cytopathic effect inhibition assay as listed

Mouse (L929/EMCV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37(2):755]. The EC₅₀ for interferon in this assay is ~2.5 U/ml. Lot Activity was derived from multiple determinations in the above assay. The units are determined by use of a Mu-IFN-beta laboratory standard calibrated to the international reference standard for mouse interferon beta provided by the National Institutes of Health [Meager, et al. (2001) J. Immunol Methods. 257 (1- 2):17-33]. Please note that IFN assays vary between labs and assay systems [Meager, et al. (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1].

Formulation, Preparation and Storage

12410-1

Formulation	20 mM Sodium Phosphate, pH 6.4; 300 mM NaCl; 5% Glycerol
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IFN-beta

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

Long Name

Interferon beta

Alternate Names

IFB, IFF, IFNB, IFNB1, IFNbeta

Entrez Gene IDs

3456 (Human); 15977 (Mouse); 24481 (Rat)

Gene Symbol

IFNB1

UniProt