Recombinant Mouse IFNAA Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10150-IF

R&D Systems, part of Bio-Techne
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10150-IF-010
10150-IF-050
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Key Product Details

Source

CHO

Accession #

Q61718

Conjugate

Unconjugated

Applications

Bioactivity

View all IFNAA Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse IFNAA protein
Cys24-Glu190

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys24

Predicted Molecular Mass

19.3 kDa

SDS-PAGE

16-21 kDa, under reducing conditions

Activity

Measured in an anti-viral assay using L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681.
The ED50 for this effect is <15 pg/mL.

Scientific Data Images for Recombinant Mouse IFNAA Protein, CF

Recombinant Mouse IFNAA Protein Bioactivity

Recombinant Mouse IFNAA Protein Bioactivity

Recombinant Mouse IFNAA (Catalog # 10150-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is <15 pg/mL.
Recombinant Mouse IFNAA Protein SDS-PAGE

Recombinant Mouse IFNAA Protein SDS-PAGE

2 μg/lane of Recombinant Mouse IFNAA (Catalog # 10150-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 16-21 kDa.

Formulation, Preparation and Storage

10150-IF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFNAA

Interferon-alpha A (IFNAA), also referred to as interferon-alpha 15 (IFNA15), is one of 14 subtypes within the IFN-alpha family (1). The members of the IFN-alpha family, also known as alpha leukocyte interferons, encompass a group of distinct but closely related proteins which share approximately 80% amino acid (aa) sequence identity and have a similar globular structure composed of five alpha-helices (1-3). IFN-alpha family members signal through a common set of cell surface receptor complex composed of IFNAR2 and IFNAR1 subunits (2). The mature extracellular domain (ECD) of mouse IFNAA is 167 aa and shares 93% and 92% aa sequence identity with mouse and rat, respectively. Most of the members of the murine IFN-alpha family are N-glycosylated, however IFNAA is one of 4 members that lacks a N-glycosylation site (3). IFNAA is one of the many interferon alpha subtypes belonging to the Type I leukocyte IFN family. The Type I IFN family has antiviral, anti-proliferative and natural killer cell activities.

Long Name

Interferon alpha A

Alternate Names

Gm12597, IFNA15

Entrez Gene IDs

242517 (Mouse)

Gene Symbol

IFNA15

UniProt

Q61718

Additional IFNAA Products

Product Documents for Recombinant Mouse IFNAA Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Manufacturing Specifications

  1. Pestka, S. (2007) J Biol Chem. 282:20047.
  2. Oritani, K. et al. (2001). Cytokine & Growth Factor Reviews, 12:337.
  3. Pesch, V. et al. (2004). Journal of Virology, 78:8219.

Product Specific Notices for Recombinant Mouse IFNAA Protein, CF

For research use only

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