Recombinant Mouse IGSF4B/SynCAM3 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9988-S4

R&D Systems, part of Bio-Techne
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9988-S4-050
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Key Product Details

Source

NS0

Accession #

Q99N28

Conjugate

Unconjugated

Applications

Bioactivity

View all IGSF4B/SynCAM3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IGSF4B/SynCAM3 protein
Asn23-His328, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn23

Predicted Molecular Mass

34 kDa

SDS-PAGE

35-44 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Mouse IGSF4B/SynCAM3, immobilized at 1.25-2.5 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.

Scientific Data Images for Recombinant Mouse IGSF4B/SynCAM3 His-tag Protein, CF

Recombinant Mouse IGSF4B/SynCAM3 His-tag Protein SDS-PAGE

Recombinant Mouse IGSF4B/SynCAM3 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse IGSF4B/SynCAM3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-44 kDa.

Formulation, Preparation and Storage

9988-S4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: IGSF4B/SynCAM3

IGSF4B (immunoglobulin superfamily member 4B), also called CADM3, TSLL1, Necl-1 and SynCAM-3, is a neural tissue-specific member of the nectin-like family of immunoglobulin superfamily (1, 2). It is a 48-50 kDa type I transmembrane (TM) glycoprotein that is concentrated at non-junctional contact sites of neuronal axons and glial processes (2, 3). In myelinated peripheral nerve fibers, IGSF4B is concentrated at nodes of Ranvier in contact sites of Schwann cells (2). The 396 amino acid (aa) mouse IGSF4B contains a 22 aa signal sequence, a 306 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 47 aa cytoplasmic domain. The IGSF4B ECD is highly conserved, sharing 95-96% aa identity between human, mouse, rat, canine and bovine sequences. The ECD of IGSF4A, B, C and D proteins share 35-50% aa identity. The ECD contains an N-terminal V-type Ig-like domain that is responsible for Ca2+‑independent homophilic and heterophilic interactions with Nectin-1, Nectin-3 or Necl-2 (IGSF-4) in trans. It also contains two C2‑type Ig-like domains that are responsible for Ca2+-independent homophilic dimerization in cis that is thought to precede trans interaction (2, 4, 5). The cytoplasmic domain binds members of the MAGUK guanylate kinase subfamily, such as Dlg3, Pals2 and CASK (2). These activities are thought to play roles in adhesion and architecture at the synapse (2). IGSF-4B was also identified as a tumor-suppressor gene and it inhibits migration, invasion, and induces differentiation of glioma cells (6). It also suppresses growth and tumorigenic ability of colon cancer cells (7). We have also shown that IGSF4B promotes in vitro outgrowth of cortical neurons.

References

  1. Fukuhara, H. et al. (2001) Oncogene 20:5401.
  2. Kakunaga, S. et al. (2005) J. Cell Sci. 118:1267.
  3. Fukami, T. et al. (2003) Gene 323:11.
  4. Shingai, T. et al. (2003) J. Biol. Chem. 278:35421.
  5. Dong, X. et al. (2006) J. Biol. Chem. 281:10610.
  6. Yin B. et al. (2009) Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 31:669.
  7. Raveh S. et al. (2009) J. Cell Biochem. 108:326.

Long Name

Immunoglobulin Superfamily, Member 4B

Alternate Names

BIgR, CADM3, Necl-1, SynCAM3, TSLL1

Entrez Gene IDs

57863 (Human); 94332 (Mouse); 360882 (Rat)

Gene Symbol

CADM3

UniProt

Q99N28

Additional IGSF4B/SynCAM3 Products

Product Documents for Recombinant Mouse IGSF4B/SynCAM3 His-tag Protein, CF

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Product Specific Notices for Recombinant Mouse IGSF4B/SynCAM3 His-tag Protein, CF

For research use only

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