Recombinant Mouse IL-18 R alpha/IL-1 R5 Fc Chimera, CF

R&D Systems, part of Bio-Techne | Catalog # 9285-BP

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

Q61098

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-18 R alpha/IL-1 R5 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IL-18 R alpha/IL-1 R5 protein
Mouse IL-18 R alpha/IL-1 R5
(Lys20-Gly326)
Accession # Q61098		 IEGRDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys20

Predicted Molecular Mass

62 kDa

SDS-PAGE

90-106 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse IL‑18 R alpha/IL‑1 R5 Chimera is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Mouse IL-18 (Catalog # 9139-CL) that produces 50% of the optimal binding response is 1.5-9 μg/mL.

Scientific Data Images for Recombinant Mouse IL-18 R alpha/IL-1 R5 Fc Chimera, CF

Recombinant Mouse IL-18 R alpha/IL-1 R5 Fc Chimera Bioactivity

Recombinant Mouse IL-18 R alpha/IL-1 R5 Fc Chimera Bioactivity

When Recombinant Mouse IL-18 Ra/IL-1 R5 Fc Chimera (Catalog # 9285-BP) is coated at 2 µg/mL, 100 µL/well, Recombinant Mouse IL-18/IL-1F4 (Catalog # 9139-IL) binds with a ED50 of 1.5-9 µg/mL.

Formulation, Preparation and Storage

9285-BP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 1 mg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-18 R alpha/IL-1 R5

IL-18 Receptor alpha (IL-18 Ra), also known as IL-18 R1, IL-1 R5, and IL-1 Rrp, is a transmembrane component of the IL-18 Receptor (1). It associates with IL-18 Rb/IL-1 R7 to form the high affinity receptor complex for IL-18 and confer cellular responsiveness to IL-18 (2, 3). IL-18 is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. Mature mouse IL-18 Ra consists of a 307 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 190 aa cytoplasmic domain (4). Within the ECD, mouse IL-18 Ra shares 56% and 82% aa sequence identity with human and rat IL-18 Ra, respectively. IL-18 Ra is widely expressed on epithelial cells as well as on B cells, NK cells, CD4+ and CD8+ T cells, and dendritic cells (5-8). Signaling through the IL-18 receptor promotes a Th1 biased immune response and NK cell cytolytic activity (6, 7, 9-11). It also contributes to cigarette smoke-induced airway inflammation and epithelial damage (12).

References

  1. Dinarello, C.A. et al. (2013) Front. Immunol. 4:289.
  2. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
  3. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
  4. Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
  5. Kunikata, T. et al. (1998) Cell Immunol. 189:135.
  6. Smeltz, R.B. et al. (2001) J. Exp. Med. 194:143.
  7. Chan, W.L. et al. (2001) J. Immunol. 167:1238.
  8. Kaser, A. et al. (2004) Blood 103:648.
  9. Tomura, M. et al. (1998) J. Immunol. 160:3759.
  10. Yoshimoto, T. et al. (1998) J. Immunol. 161:3400.
  11. Hyodo, Y. et al. (1999) J. Immunol. 162:1662.
  12. Kang, M.J. et al. (2007) J. Immunol. 178:1948.

Long Name

Interleukin 18 Receptor alpha

Alternate Names

CD218a, IL-1 R5, IL-1 Rrp, IL-18 R1, IL-18Ra, IL18R alpha, IL18R1

Entrez Gene IDs

8809 (Human); 16182 (Mouse)

Gene Symbol

IL18R1

UniProt

Q61098

Additional IL-18 R alpha/IL-1 R5 Products

Product Documents for Recombinant Mouse IL-18 R alpha/IL-1 R5 Fc Chimera, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse IL-18 R alpha/IL-1 R5 Fc Chimera, CF

For research use only

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