Recombinant Mouse IL-27 p28/IL-30 Protein

R&D Systems, part of Bio-Techne | Catalog # 7430-ML

R&D Systems, part of Bio-Techne
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Carrier Free
7430-ML-010/CF
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With Carrier
7430-ML-010
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Key Product Details

Source

NS0

Accession #

Q8K3I6

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-27 p28/IL-30 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IL-27 p28/IL-30 protein
Phe29-Ser234, with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe29

Predicted Molecular Mass

24.9 kDa

SDS-PAGE

25-35 kDa, reducing conditions

Activity

Measured by its ability to induce CXCL10/IP-10 secretion by THP-1 human acute monocytic leukemia cells.
The ED50 for this effect < 35 ng/mL.

Reviewed Applications

Read 1 review rated 5 using 7430-ML in the following applications:

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 7430-ML
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 7430-ML/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl and CHAPS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-27 p28/IL-30

Interleukin‑27 p28, also known as IL‑30, is a secreted 28 kDa protein that is considered a member of the IL‑6/IL‑12 interfamily cytokine family. p28/IL‑30 is one of several alpha‑chain proteins that can associate with various beta‑chain proteins to form heterodimeric cytokines in these families. The alpha-chains (e.g. IL‑6, IL‑11, Cardiotrophin‑1, CLC/CNTF, LIF, Oncostatin M, IL‑23 p19, IL‑27 p28/IL‑30, IL‑12/IL‑35 p35) have a four‑helix bundle structure, while the beta‑chains (e.g. EBI‑3, CLF‑1, IL‑12/IL‑23 p40) resemble class 1 cytokine receptors. These cytokines utilize heteromeric cell surface receptors which contain shared as well as ligand‑specific subunits. Divergent biological responses are obtained from the combinatorial association of cytokine subunits and their interaction with various combinations of receptor subunits. Complexity in this system is increased by the generation of soluble receptors and by the competition between proteins for shared subunit pairing (1‑3). p28/IL‑30 is expressed by macrophages and dendritic cells, and is up‑regulated in these cells by inflammatory stimuli (4‑9). It was first described as a partner with EBI‑3 in the heterodimeric cytokine IL‑27 (4). IL‑27 signals through a receptor complex composed of IL‑27 R alpha/WSX-1/TCCR and gp130 (4, 10, 11). This interaction enhances the proinflammatory activation of naïve CD4+ T cells, NK cells, mast cells, and monocytes and the cytotoxic activity of CD8+ T cells (4, 10, 12). IL‑27 also exhibits anti-inflammatory activity, including the induction of IL‑10 production by naïve and memory T cells, the activation of regulatory T cells (Treg), and the suppression of Th17 cytokine secretion (13, 14). Alternatively, p28/IL‑30 associates with CLF‑1 to create a cytokine that triggers responses through IL‑27 R alpha, IL‑6 R alpha, and gp130 (6). Like IL‑27, p28‑CLF-1 heterodimers co‑stimulate IFN‑ gamma production by NK cells, and induce IL‑10 secretion by CD4+ T cells (6). In contrast to IL‑27, however, p28‑CLF-1 is reported to promote the differentiation of Th17 cells (6). A third mode of p28 action enables it to stimulate cells that express both IL‑6 R alpha and gp130, but lack IL‑27 R alpha (11). Similar to the IL‑6 system, the presence of IL‑6 R alpha on the cell surface is not even required if p28/IL‑30 associates with a soluble form of IL‑6 R alpha. This combination can trigger trans signaling through gp130, a mechanism that has been demonstrated for complexes of IL‑6 with soluble IL‑6 R alpha (11). Over‑expression of p28/IL‑30 in vivo interferes with humoral antibody responses and protects from IL‑12 induced liver inflammation (7, 15). Mature mouse p28/IL-30 shares 70% and 89% amino acid sequence identity with human and rat p28, respectively

References

  1. Hunter, C.A. and R. Kastelein (2012) Immunity 37:960.
  2. Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85.
  3. Vignali, D.A.A. and V.K. Kuchroo (2012) Nat. Immunol. 13:722.
  4. Pflanz, S. et al. (2002) Immunity 16:779.
  5. Liu, J. et al. (2007) J. Exp. Med. 204:141.
  6. Crabé, S. et al. (2009) J. Immunol. 183:7692.
  7. Stumhofer, J.S. et al. (2010) Nat. Immunol. 11:1119.
  8. Bosmann, M. et al. (2012) J. Immunol. 188:5086.
  9. Dibra, D. et al. (2012) J. Immunol. 188:3709.
  10. Pflanz, S. et al. (2004) J. Immunol. 172:2225.
  11. Garbers, C. et al. (2013) J. Biol. Chem. 288:4346.
  12. Schneider, R. et al. (2011) Eur. J. Immunol. 41:47.
  13. Diveu, C. et al. (2009) J. Immunol. 182:5748.
  14. Hall, A.O, et al. (2012) Immunity 37:511.
  15. Dibra, D. et al. (2012) Hepatology 55:1204.

Long Name

Interleukin 27 p28

Alternate Names

IL-30, IL27 p28

Entrez Gene IDs

246778 (Human); 246779 (Mouse)

Gene Symbol

IL27

UniProt

Q8K3I6

Additional IL-27 p28/IL-30 Products

Product Documents for Recombinant Mouse IL-27 p28/IL-30 Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse IL-27 p28/IL-30 Protein

For research use only

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