Recombinant Mouse IL-27 Ra/WSX-1/TCCR Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2109-TC

R&D Systems, part of Bio-Techne
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2109-TC-050
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Key Product Details

Source

NS0

Accession #

O70394

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-27 R alpha/WSX-1/TCCR Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IL-27 R alpha/WSX-1/TCCR protein
Mouse IL-27 R alpha
(Gly29-Lys510)
Accession # O70394		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly29

Predicted Molecular Mass

80 kDa (monomer)

SDS-PAGE

105-115 kDa, reducing conditions

Activity

Measured by its ability to inhibit IL-27 anti-viral activity using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Bender H. et al. (2009) Hepatology 50:585.
The ED50 for this effect is 2-12 µg/mL.

Formulation, Preparation and Storage

2109-TC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-27 R alpha/WSX-1/TCCR

IL‑27 R alpha (also known as WSX‑1 and TCCR) is a 85‑95 kDa member of the type I, group 2 cytokine receptor family (1‑6). Mature IL‑27 R alpha is a type I transmembrane glycoprotein that contains a 486 amino acid (aa) extracellular region, a 21 aa transmembrane segment and a 92 aa cytoplasmic domain. Consistent with type I cytokine receptors, the extracellular region contains four positionally conserved cysteine residues, a WSxWS motif (for receptor folding and ligand binding), and three fibronectin type III repeats. The intracellular domain contains a “box‑1” motif that may be involved with Janus kinases (3). In mouse, a soluble 33 kDa splice form that shows a 20 aa substitution for aa 251‑623 has been identified (7). The mouse IL‑27 R alpha extracellular region shares 63% amino acid identity with the human IL‑27 R alpha extracellular domain (2, 3). IL‑27 R alpha is expressed in mast cells, endothelial cells, NK cells, macrophages, monocytes, B cells, dendritic cells, and naïve T cells (1, 2, 4, 8). Typical of other class I cytokine receptor chains, the ligand binding IL‑27 R alpha molecule is known to heterodimerize with a signal‑transducing subunit (gp130) to form a functional IL‑27 receptor (9, 10). In addition, IL‑27 R alpha is reported to complex with CNTFR alpha and gp130 form a humanin receptor on neurons (7, 11), and to complex with gp130 and IL‑6 R to form a receptor for a p28:CLF heterodimeric cytokine on lymphocytes (12). Studies using IL‑27 R alpha/WSX‑1-/- mice reveal that IL‑27 has the ability to suppress T cell activity during infection, and to mediate an inhibition of both type 1 and type 2 T cell immunity (4, 13, 14). In particular, IL‑27 is known to act on naïve T cells, blocking their differentiation into a Th17 phenotype. Notably, cells committed to a Th17 phenotype, although they express a functional IL‑27 receptor, are unresponsive to the effects of IL‑27 (15). Activated T cells that are CD4+ and CD8+, and which express the IL‑27 receptor, can be induced by IL‑27 to form a double‑positive CD25+ FoxP3- IFN‑ gamma plus IL‑10 secreting phenotype that both promotes and suppresses the inflammatory response (16).

References

  1. Villarino, A.V. et al. (2004) J. Immunol. 173:715.
  2. Chen, Q. et al. (2000) Nature 407:916.
  3. Sprecher, C.A. et al. (1998) Biochem. Biophys. Res. Commun. 246:82.
  4. Artis, D. et al. (2004) J. Immunol. 173:5626.
  5. Yoshida, H. and Y. Miyazaki (2008) Int. J. Biochem. Cell Biol. 40:2379.
  6. Yoshida, H. and M. Yoshiyuki (2008) Immunol. Rev. 226:234.
  7. Hashimoto, Y. et al. (2009) Biochem. Biophys. Res. Commun. 389:95
  8. Holscher, C. et al. (2005) J. Immunol. 174:3534.
  9. Pflanz, S. et al. (2004) J. Immunol. 172:2225.
  10. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
  11. Hashimoto, Y. et al. (2009) Mol. Biol. Cell 20:2864.
  12. Crabe, S. et al. (2009) J. Immunol. 183:7692.
  13. Villarino, A. et al. (2003) J. Immunol. 170:645.
  14. Hamano., S. et al. (2003) Immunity 19:657.
  15. El-behi, M. et al. (2009) J. Immunol. 183:4957.
  16. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.

Long Name

Interleukin-27 Receptor Subunit alpha

Alternate Names

IL-27 R alpha, IL-27Ra, IL27R alpha, IL27RA, TCCR, WSX-1

Entrez Gene IDs

9466 (Human); 50931 (Mouse)

Gene Symbol

IL27RA

UniProt

O70394

Additional IL-27 R alpha/WSX-1/TCCR Products

Product Documents for Recombinant Mouse IL-27 Ra/WSX-1/TCCR Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse IL-27 Ra/WSX-1/TCCR Fc Chimera Protein, CF

For research use only

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