Recombinant Mouse IL-Y Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9989-IL

R&D Systems, part of Bio-Techne
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9989-IL-050
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Key Product Details

Source

NS0

Accession #

P43432, Q8K3I6

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-Y (IL-12p40/IL-27p28) Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IL-Y (IL-12p40/IL-27p28) protein
Mouse IL-12p40
(Met23-Ser335)
Accession # P43432		 GGGSGGGSGGGS Mouse IL-27p28
(Phe29-Ser234)
Accession # Q8K3I6
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met23 (IL-12p40)

Predicted Molecular Mass

60 kDa

SDS-PAGE

74-84 kDa, reducing conditions

Activity

Measured by its ability to inhibit IL-12 induced IFN-gamma secretion by mouse splenocytes.
The ED50 for this effect is 10-60 ng/mL.

Scientific Data Images for Recombinant Mouse IL-Y Protein, CF

Recombinant Mouse IL-Y Protein Bioactivity

Recombinant Mouse IL-Y Protein Bioactivity

Recombinant Mouse IL-Y (Catalog # 9989-IL) inhibits IL‑12 induced IFN-gamma secretion in mouse splenocytes. The ED50 for this effect is 10‑60 ng/mL.
Recombinant Mouse IL-Y Protein SDS-PAGE

Recombinant Mouse IL-Y Protein SDS-PAGE

2 μg/lane of Recombinant Mouse IL-Y was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 74-84 kDa.

Formulation, Preparation and Storage

9989-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: IL-Y (IL-12p40/IL-27p28)

IL-Y is a new member of the IL-12 cytokine family consists of IL-12p40 and IL-27p28 subunits (1, 2, 3). The mature mouse IL-12p40 subunit contains 312 amino acids and shares 66% and 93%amino acid (aa) identity with human and rat IL-12p40, respectively. The mature mouse IL-27p28 subunit contains 205 amino acids and shares 70% and 89% aa identity with human and rat IL-27p28, respectively. IL-Y inhibits the differentiation and inflammatory responses of Th1 and Th17 cells while promoting expansion of IL-10 and Foxp3 expressing regulatory T cells (3). Treatment of prediabetic non-obese diabetic mice using adenovirus vector expressing IL-Y prevents the onset of hyperglycemia with reduced expression of inflammatory mediators such as IFN-gamma. IL-Y significantly stimulates a unique cytokine and chemokine expression profile as well as to activate STAT3, in part, through a pathway involving WSX-1 (2).

References

  1. Hasegawa, H. et al. (2016) Frontiers in Immunology 7:479.
  2. Flores, R. et al. (2015) Eur J Immunol. 45:3114.
  3. Wang, R. et al. (2012) The Journal of Biological Chemistry 287:36012.

Long Name

Interleukin Y

Entrez Gene IDs

246778 (Human)

Gene Symbol

IL27

UniProt

P43432, Q8K3I6

Additional IL-Y (IL-12p40/IL-27p28) Products

Product Documents for Recombinant Mouse IL-Y Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse IL-Y Protein, CF

For research use only

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