Recombinant Mouse Inhibin A Protein

R&D Systems, part of Bio-Techne | Catalog # 8346-IN

R&D Systems, part of Bio-Techne
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Carrier Free
8346-IN-010/CF
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With Carrier
8346-IN-010
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Key Product Details

Source

CHO

Accession #

CAA49324 ( alpha chain) & P08476 ( beta chain)

Structure / Form

Disulfide-linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Inhibin A Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Inhibin A protein
Ser234-Ile366 ( alpha chain) & Gly311-Ser426 ( beta chain)

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser234 ( alpha chain) & Gly311 ( beta chain)

Predicted Molecular Mass

14.5 kDa ( alpha chain) & 13.0 kDa ( beta chain)

SDS-PAGE

12-14 kDa & 17-20 kDa, reducing conditions

Activity

Measured by its ability to neutralize Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells.
The ED50 for this effect is 5-30 ng/mL.

Scientific Data Images for Recombinant Mouse Inhibin A Protein

Recombinant Mouse Inhibin A Protein Binding Activity

Recombinant Mouse Inhibin A Protein Binding Activity

Recombinant Mouse Inhibin A (Catalog # 8346-IN) neutralizes Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 5-30 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 8346-IN
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 8346-IN/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Inhibin A

Inhibin A is a member of the TGF-beta superfamily of proteins (1). Mature Inhibin A is a disulfide-linked dimer composed of alpha and beta subunits (1). Inhibin A shares a common alpha subunit with the closely related protein Inhibin B but has a unique beta subunit ( betaA) (2-4). The mature alpha subunit of mouse Inhibin A has a predicted molecular weight of 14.7 kDa and shares 80% and 97% amino acid (aa) sequence identity with the human and rat orthologs, respectively. The mature beta subunit of this mouse protein has a predicted molecular weight of 13 kDa and shares 93% and 100% aa sequence identity with the human and rat orthologs, respectively. Inhibin binds and antagonizes ActRIIA and ActRIIB in complex with the TGF-beta RIII and/or IGSF1 co-receptors and subsequently acts to suppress Activin-induced Follicle Stimulating Hormone (FSH) secretion (1, 5-7). Inhibins are produced by gonadal cells in both males and females (8). They are thought to be involved in the regulation of gametogenesis, and embryonic and fetal development (8, 9). Elevated concentrations of Inhibins are associated with pregnancy, preeclampsia, and ovarian cancer, and Inhibin A levels are typically measured during prenatal screening for Down’s syndrome (10-14).

References

  1. Phillips, D.J. and T.K. Woodruff (2004) Growth Factors 22:13.
  2. Ling, N. et al. (1985) Proc. Natl. Acad. Sci. USA 82:7217.
  3. Robertson, D.M. et al. (1985) Biochem. Biophys. Res. Commun. 126:220.
  4. Mason, A.J. et al. (1986) Biochem. Biophys. Res. Commun. 135:957.
  5. Lewis, K.A. et al. (2000) Nature 404:411.
  6. Martens, J.W. et al. (1997) Endocrinology 138:2928.
  7. Chapman, S.C. et al. (2002) Mol. Cell. Endocrinol. 196:79.
  8. de Kretser, D.M. and D.M. Robertson (1989) Biol. Reprod. 40:33.
  9. Knight, P.G. et al. (2012) Mol. Cell. Endocrinol. 359:53.
  10. Walentowicz, P. et al. (2014) PLoS One 9:e90575.
  11. Kondi-Pafiti, A. et al. (2013) Clin. Exp. Obstet. Gynecol. 40:109.
  12. Kuijper, E.A. et al. (2013) Reprod. Biomed. Online 27:33.
  13. Carty, D.M. et al. (2008) Trends Cardiovasc. Med. 18:186.
  14. Wald, N.J. et al. (1996) Prenat. Diagn. 16:143.

Alternate Names

INHA, Inhibin A

Entrez Gene IDs

3623 (Human); 16322 (Mouse)

Gene Symbol

INHA

UniProt

CAA49324 ( alpha chain) & P08476 ( beta chain)

Additional Inhibin A Products

Product Documents for Recombinant Mouse Inhibin A Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 8346-IN (with carrier)
Download SDS 8346-IN/CF (carrier free)

Product Specific Notices for Recombinant Mouse Inhibin A Protein

For research use only

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