Recombinant Mouse Integrin alpha 1 beta 1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8188-AB

R&D Systems, part of Bio-Techne
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8188-AB-025
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Key Product Details

Source

CHO

Accession #

Q3V3R4(Integrin alpha1) & P09055 (Integrin beta1)

Structure / Form

Noncovalently-linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Integrin alpha 1 beta 1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha 1 beta 1 protein
Mouse Integrin alpha1
(Phe29-Pro1141)
Accession # Q3V3R4		 His-Pro GGGSGGGS Acidic Tail 6-His tag
Mouse Integrin beta1
(Gln21-Asp728)
Accession # P09055		 His-Pro GGGSGGGS Basic Tail
N-terminus C-terminus

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe29 (Integrin alpha1) & No results obtained: Gln21 predicted (Integrin beta1), sequencing might be blocked

Predicted Molecular Mass

132 kDa (Integrin alpha1) & 86.5 kDa (Integrin beta1)

SDS-PAGE

120-135 kDa & 180-215 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Collagen I is immobilized at 5 μg/mL, Recombinant Mouse Integrin alpha1 beta1 can bind with an apparent Kd <5 nM.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

8188-AB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 1 beta 1

Integrin alpha1 beta1, also called VLA1, is the only alpha1 integrin family adhesion receptor, one of twelve integrins that share the beta1 subunit, and one of four collagen-binding integrins (1-6). It is the non-covalent heterodimer of 190-210 kDa alpha1 (CD49a) and 130 kDa beta1 (CD29) type I transmembrane glycoprotein subunits. It is found on cells including activated T cells, B cells, monocytes, vascular smooth muscle cells, osteoblasts and adipocytes (2, 7-9). The alpha1 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 4). The beta1 ECD contains a vWFA domain, which participates in binding (3). Each subunit then has a transmembrane sequence and a short cytoplasmic tail. Divalent cations and intracellular (inside-out) signaling convert the dimer from the folded, inactive form to its most active, extended conformation (1, 2). The 1113 amino acid (aa) mouse alpha1 extracellular domain (ECD) shares 88% and 96% aa sequence identity with human and rat alpha1, respectively, while the 708 aa mouse beta1 ECD shares 93% and 98% aa sequence identity with human and rat beta1, respectively. alpha1 beta1 preferentially binds collagens I, IV, VI, XIII and XVI, but also binds laminin (4-11). alpha1 beta1 is reported to down-regulate EGF R signaling, increase expression of caveolin-1, reduce production of reactive oxygen species, regulate collagen expression, control MMP collagenase and gelatinase activity, and mediate the renal basement membrane disorder Alport syndrome (11-13). These effects may begin by alpha1 beta1 binding of caveolin-1, initiating signaling pathways that involve the phosphatase TC-PTP, kinases ERK and p38, and the transcription factor PPAR-gamma (11-14). alpha1 beta1 down-regulates MMP-mediated angiostatin formation, enhancing tumor vascularization (9). alpha1 beta1-null mice are deficient in fibroblast collagen IV and laminin-mediated cell spreading and migration, show defects in bone healing, and are resistant to Alport renal fibrosis (10-12, 15). When expressed in the same epithelial cells, alpha1 beta1 negatively regulates integrin alpha2 beta1-mediated cell adhesion and migration (16).

References

  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Argraves, W.S. et al. (1987) J. Cell Biol. 105:1183.
  4. Briesewitz, R. et al. (1993) J. Biol. Chem. 268:2989.
  5. Holers, V.M. et al. (1989) J. Exp. Med. 169:1589.
  6. Tulla, M. et al. (2001) J. Biol. Chem. 276:48206.
  7. Eble, J.A. et al. (2006) J. Biol. Chem. 281:25745.
  8. Hall, D.E. et al. (1990) J. Cell Biol. 110:2175.
  9. Pozzi, A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2202.
  10. Gardner, H. et al. (1999) J. Cell Sci. 112:263.
  11. Dennis, J. et al. (2010) Am. J. Pathol. 177:2527.
  12. Cosgrove, D. et al. (2008) Am. J. Pathol. 172:761.
  13. Chen, X. et al. (2010) Mol. Cell. Biol. 30:3048.
  14. Borza, C.M. et al. (2010) J. Biol. Chem. 285:40114.
  15. Gardner, H. et al. (1996) Dev. Biol. 175:301.
  16. Abair, T.D. et al. (2008) Exp. Cell Res. 314:3593.

Entrez Gene IDs

3672 (Human)

Gene Symbol

ITGA1

UniProt

Q3V3R4(Integrin alpha1) & P09055 (Integrin beta1)

Additional Integrin alpha 1 beta 1 Products

Product Documents for Recombinant Mouse Integrin alpha 1 beta 1 Protein, CF

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