Recombinant Mouse Integrin alpha V beta 8 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8314-AV

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
8314-AV-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

CHO

Accession #

P43406 & Q0VBD0

Structure / Form

Non-covalent heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Integrin alpha V beta 8 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha V beta 8 protein
Mouse Integrin aV
(Phe31-Val988)
Accession # P43406		 His-Pro GGGSGGGS Acidic Tail 6-His tag
Mouse Integrin beta8
(Glu43-Ser679)
Accession # Q0VBD0		 His-Pro GGGSGGGS Basic Tail
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe31 ( alphaV subunit) & Glu43 ( beta8 subunit) 

Predicted Molecular Mass

115 kDa ( alphaV subunit), 78 kDa ( beta8 subunit)

SDS-PAGE

95 - 170 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Mouse Integrin alphaV beta8 at 2 μg/mL can bind Recombinant Human LAP (TGF-beta 1) (Catalog # 246-LP) with an apparent Kd <0.1 nM.

Formulation, Preparation and Storage

8314-AV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha V beta 8

Integrin  alphaV beta8 is a type I transmembrane non-covalent heterodimer composed of a 115 kDa alphaV/CD51 subunit and an 84 kDa beta8 subunit. The Integrin  beta8 exclusively partners with the alphaV subunit, while alphaV forms heterodimers with beta1, beta3, beta5 and beta6 (1). Integrin  alphaV beta8 is expressed on Schwann cells and astrocytes in the brain, vascular epithelial cells, mesangial cells in the kidney, and fibroblasts and epithelial cells in the airway (2-7). Interestingly, Integrin alphaV beta8 is expressed by cells of the immune system, most prominently on CD4+ T cells and on dendritic cells (8). Unlike other alphaV integrins, alphaV beta8 does not interact with the cytoskeleton or activate cytoplasmic signaling pathways (3, 9). Instead, it binds ligands containing an arginine-glycine-aspartic acid (RGD) motif, including Vitronectin, Fibrin and the latency associated peptide (LAP) (10, 11). High affinity binding of alphaV beta8 to LAP triggers the MT1-MMP induced proteolytic cleavage of LAP and the release of active TGF-beta (12). Active TGF-beta regulates cell growth and nearby vascularization (5, 12, 13). Furthermore, Integrin alphaV beta8-mediated TGF-beta activation in specialized dendritic cells of the intestine is crucial for maintaining immune homeostasis in the gut (14). Deletion of either alphaV or beta8 reveals that alphaV beta8 is required for vascular morphogenesis in the embryonic brain (15-16). The 958 amino acid mouse alphaV extracellular domain (ECD) shares 92% and 88% aa sequence identity with human and rat alphaV, respectively, while the 637 aa mouse beta8 ECD shares 87% and 96% aa sequence identity with human and rat orthologs, respectively.

References

  1. Luo, BH. et al. (2007) Annu.Rev.Imm.25:619.
  2. Milner, R. et al. (1997) Glia 21:350.
  3. Nishimura, S. et al. (1998) Brain Res. 791:271.
  4. Zhu, J. et al. (2002) Development 129:2891.
  5. Cambier, S. et al. (2000) Cancer Res. 60:7084.
  6. Khan, S. et al. (2011) Am. J. Pathol. 178:609.
  7. Araya, J. et al. (2006) Am. J. Pathol. 169:405.
  8. Travis, M.A. et al. (2007) Nature 449:361.
  9. Moyle, M. et al. (1991) J. Biol. Chem. 266:19650.
  10. Nishimura, S. et al. (1994) J. Biol. Chem. 269:28708.
  11. Chernousov, M. A. and D. J. Carey (2003) Exp. Cell Res. 291:514.
  12. Mu, D. et al. (2002) J. Cell Biol. 157:493.
  13. Araya, J. et al. (2006) Am. J. Pathol. 169:405.
  14. Worthington, J.J. et al. (2012) Immunobiology 217:1259.
  15. Cambier, S. et al. (2005) Am. J. Pathol. 166:1883.
  16. Proctor, J. M. et al. (2005) J. Neurosci. 25:9940.
  17. McCarty, J. H. et al. (2005) Development 132:165.

Entrez Gene IDs

3685 (Human)

Gene Symbol

ITGAV

UniProt

P43406 & Q0VBD0

Additional Integrin alpha V beta 8 Products

Product Documents for Recombinant Mouse Integrin alpha V beta 8 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse Integrin alpha V beta 8 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.