Recombinant Mouse Integrin alpha X beta 2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7987-AX

R&D Systems, part of Bio-Techne
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7987-AX-050
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Key Product Details

Source

CHO

Accession #

Q9QXH4 (Integrin alphaX) & P11835 (Integrin beta2)

Structure / Form

Noncovalently-linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Integrin alpha X beta 2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha X beta 2 protein
Mouse Integrin alphaX
(Phe20-Pro1116)
Accession # Q9QXH4		 His-Pro GGGSGGGS Acidic Tail 6-His tag
Mouse Integrin beta2
(Gln24-Asn702)
Accession # P11835		 His-Pro GGGSGGGS Basic Tail
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe20 (Integrin alphaX) & Gln24 predicted, No results obtained: sequencing might be blocked (Integrin beta2)

Predicted Molecular Mass

131 kDa (Integrin alphaX) & 83 kDa (Integrin beta2)

SDS-PAGE

135-150 kDa & 95-105 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support adhesion of J45.01 human acute lymphoblastic leukemia T lymphocytes.
The ED50 for this effect is 0.750-7.50 μg/mL.

Formulation, Preparation and Storage

7987-AX
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha X beta 2

Integrin alphaX beta2, also called CD11c/CD18, p150/95 or complement receptor type 4 (CR4), is one of four beta2 integrins. The non-covalent heterodimer of 150 kDa alphaX/CD11c and 95 kDa beta2/CD18 integrin subunits is commonly used as a marker for dendritic cells (DC) and classically activated macrophages (M1), but is also expressed on hairy cell leukemias, with lower amounts on other myeloid cells and activated B, NK and some cytotoxic T cells (1‑7). Inflammation enhances surface expression of alphaX beta2 in states such as obesity (adipose DC), asthma (lung DC) and hypertriglyceridemia (circulating monocytes), increasing the adhesive capacity of the cells and contributing to pathology (8‑10). The alphaX vWFA or I‑domain, which contains the adhesion sites, forms the N‑terminal head region with the alphaX beta-propeller and the beta2 vWFA domain (1, 11). Like other integrins, alphaX beta2 has multiple activation states (3). In the presence of divalent cations and "inside-out" signaling, alphaX beta2 is fully active and extended. In the inactive state, the heterodimer flexes in the center at the alphaX thigh and calf domains and beta2 I‑EGF domains, impeding access to adhesion sites (1). The 1097 aa mouse alphaX/CD11c ECD shares 87% aa sequence identity with rat, and 71‑73% with human, canine and equine alphaX, while the 679 aa mouse beta2/CD18 ECD shares 91% aa sequence identity with rat, and 80-82% with human, bovine, canine, and porcine beta2 ECD. Active alphaX beta2 shares some adhesion partners with alphaM beta2/CD11b/CD18, including complement opsonin fragment iC3b, ICAMs, vWF and fibrinogen, and is expressed on many of the same cells (4-14). However, alphaM beta2 activity is often constitutive, while alphaX beta2 activity requires cell activation (4-7). alphaX beta2 also binds osteopontin, Thy-1, plasminogen, heparin, and proteins with abnormally exposed acidic residues (14-18). The adhesion events are important for proliferation, degranulation, chemotactic migration, and phagocytosis of complement-opsonized particles (5, 6, 12, 14, 15). Mutations of beta2, especially in the vWFA domain, cause leukocyte adhesion deficiency (LAD-1) and susceptibility to bacterial infections (19).

References

  1. Corbi, A.L. et al. (1987) EMBO J. 6:4023.
  2. Kishimoto, T.K. et al. (1987) Cell 48:681.
  3. Hynes, R.O. (2002) Cell 110:673.
  4. Arnaout, M.A. (1990) Blood 75:1037.
  5. Postigo, A.A. et al. (1991) J. Exp. Med. 174:1313.
  6. Beyer, M. et al. (2005) Respir. Res. 6:70.
  7. Nicolaou, F. et al. (2003) Blood 101:4033.
  8. Stefanovic-Racic, M. et al. (2012) Diabetes 61:2330.
  9. Gower, R.M. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:160.
  10. van Rijt, L.S. et al. (2005) J. Exp. Med. 201:981.
  11. Vorup-Jensen, T. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1873.
  12. Bilsland, C.A.G. et al. (1994) J. Immunol. 152:4582.
  13. Pendu, R. et al. (2006) Blood 108:3746.
  14. Sadhu, C. et al. (2007) J. Leukoc. Biol. 81:1395.
  15. Schack, L. et al. (2009) J. Immunol. 182:6943.
  16. Choi, J. et al. (2005) Biochem. Biophys. Res. Commun. 331:557.
  17. Gang, J. et al. (2007) Mol. Cells 24:240.
  18. Vorup-Jensen, T. et al. (2007) J. Biol. Chem. 282:30869.
  19. Kishimoto, T.K. et al. (1987) Cell 50:193.

Entrez Gene IDs

3687 (Human)

Gene Symbol

ITGAX

UniProt

Q9QXH4 (Integrin alphaX) & P11835 (Integrin beta2)

Additional Integrin alpha X beta 2 Products

Product Documents for Recombinant Mouse Integrin alpha X beta 2 Protein, CF

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Product Specific Notices for Recombinant Mouse Integrin alpha X beta 2 Protein, CF

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