Recombinant Mouse Kininogen HMW (HKa) Protein, CF

Kininogen, also known as alpha2-thiol proteinase inhibitor, is a multi-function protein. There are two alternatively spliced forms, designated as the high molecular weight (HMW) and low MW (LMW) forms (1). The mouse HMW form is synthesized as a 661 amino acid residue precursor with a signal peptide (residues 1-20). The mature chain (residues 21-661) is further processed into the heavy (residues 21‑379) and the light (residues 389-661) chains. The active peptide bradykinin (residues 380-388) is released, which has a variety of functions including muscle contraction, hypotension and inflammation. The heavy chain consists of three cystatin-like domains, which are responsible for inhibiting cysteine proteases. The light chain consists of a His-rich domain, which is associated with the clotting activity. In comparison to the HMW form, the LMW Kininogen (432 residues) has the same sequence in its heavy chain and bradykinin, but a different sequence in its light chain (residues 401‑432). The LMW form is not involved in blood clotting.