Recombinant Mouse L1CAM Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5674-NC

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

P11627

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all L1CAM Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse L1CAM protein
Mouse L1CAM
(Met1-Glu1123) with a Phe176Leu Substitution
Accession # P11627		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile20

Predicted Molecular Mass

150.5 kDa (monomer)

SDS-PAGE

180‑200 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells.
Recombinant Mouse L1CAM Fc Chimera immobilized at 2.5 µg/mL with 100 µL/well will induce  >40% cell adhesion.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

5674-NC
Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: L1CAM

Neural cell adhesion molecule L1 (NCAM‑L1; also L1‑CAM, L1, and CD171) is a 200 ‑ 220 kDa type I transmembrane glycoprotein of the immunoglobulin superfamily and L1/neurofascin/NgCAM family (1, 2). NCAM-L1 is expressed by neurons, especially by growing axons on their growth cones (2). Non-neuronal cells such as Schwann cells, astrocytes, epithelial cells, and cells of myelomonocytic and lymphoid origin also express NCAM-L1 (2). Full length mouse NCAM-L1 is synthesized as a 1260 amino acid (aa) precursor that contains a 19 aa signal sequence, a 1104 aa extracellular domain (ECD), a 23 aa transmembrane region, and a 114 aa cytoplasmic tail that is highly conserved (3). The ECD contains six Ig-like C2-type domains, five fibronectin type III domains, and 21 potential sites for N‑linked glycosylation. Mouse NCAM-L1 shares 88% aa sequence identity with human NCAM-L1. NCAM-L1 is critical for neural development.  Specifically, NCAM-L1 plays a key role in neuronal cell migration, axon outgrowth, axon fasciculation, synaptogenesis, and myelination (4). NCAM-L1 mediates hemophilic cell-cell interaction but also binds heterophilic ligands like axonin-1, CD24, CD9, neurocan and several integrins (4). It has been shown that NCAM-L1 can undergo membrane-proximal cleavage by ADAM10 and ADAM17, leading to the release of the soluble extracellular domain and the generation of a membrane-bound stub (4). Remaining intact, the soluble extracellular domain has been suggested to serve as a substrate for integrin-mediated cell adhesion, thereby stimulating cellular motility and cell migration (4). It has also been found that NCAM‑L1 plays a role in the ontogeny of human tumors and its expression is linked to poor prognosis (1). Over‑expression promotes tumor-cell invasion and motility, growth in nude mice and tumor metastasis (1). Research shows that proteolytic processing by ADAM10 and presenilin/ gamma-secretase is essential for the nuclear signaling of NCAM-L1 in human carcinoma cell lines (1). Defects in NCAM-L1 are the cause of MASA syndrome (mental retardation, aphasia, shuffling gait and adducted thumbs), which is also known as CRASH syndrome (corpus callosum hypoplasia, psychomotor retardation, adducted thumbs, spastic paraparesis and hydrocephalus (5, 6).

References

  1. Riedle, S. et al. (2009) Biochem. J. 420:391.
  2. Kenwrick, S. and P. Doherty (1998) Bioessays 20:668.
  3. Crossin, K.L. and L.A. Krushel (2000) Dev. Dyn. 218:260.
  4. Maretzky, T. et al. (2005) Mol. Cell. Biol. 25:9040.
  5. Kamiguchi, H. et al. (1998) Mol. Cell Neurosci. 12:48.
  6. Striha, L. et al. (2000) J. Child Neurol. 15:239.

Long Name

Cell Adhesion Molecule L1

Alternate Names

CAML1, CD171, HSAS, HSAS1, MASA, MIC5, NCAM-L1, S10, SPG1

Entrez Gene IDs

3897 (Human); 16728 (Mouse); 50687 (Rat)

Gene Symbol

L1CAM

UniProt

P11627

Additional L1CAM Products

Product Documents for Recombinant Mouse L1CAM Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse L1CAM Fc Chimera Protein, CF

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