Recombinant Mouse LAIR1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4016-LR

R&D Systems, part of Bio-Techne
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4016-LR-050
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Key Product Details

Source

NS0

Accession #

Q8BG84

Conjugate

Unconjugated

Applications

Bioactivity

View all LAIR1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse LAIR1 protein
Gln22-Tyr141 & Ser25-Tyr141, both with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser25 & No results obtained: Gln22 predicted

Predicted Molecular Mass

14.3 kDa

SDS-PAGE

20-35 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of HT-29 human colon adenocarcinoma cells.
When 5 x 104 cells/well are added to Recombinant Mouse LAIR1-coated plates (50 µg/mL with 100 µL/well), approximately 30‑60% will adhere after 10 minutes at 37° C.
Optimal concentration depends on cell type as well as the application or research objectives.

Reviewed Applications

Read 2 reviews rated 4 using 4016-LR in the following applications:

Formulation, Preparation and Storage

4016-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAIR1

Leukocyte-associated Ig-like receptor-1 (LAIR1) is an inhibitory receptor of the Ig superfamily that is structurally related to inhibitory members of KIR and ILT/CD85 families (1-3). It is expressed on immune cells, including NK cells, T cells, B cells, monocytes, immature neutrophils, dendritic cells and most thymocytes (2-4). The 253 amino acid (aa) type I transmembrane (TM) protein contains a 21 aa signal sequence, a 124 aa extracellular domain (ECD), a 20 aa TM domain and a 98 aa cytoplasmic domain. The ECD includes one C2-type Ig-like domain and two potential N-linked glycosylation sites. Tyrosine phosphorylation of two cytoplasmic ITIM motifs results in recruitment of phosphatases and down-regulation of signaling through activating receptors (2, 3, 5). Crosslinking of LAIR1 inhibits processes such as B cell receptor-mediated activation, NK cell and T cell cytotoxicity and basophil degranulation (1-3). Four mouse LAIR1 splice variants have been identified, but it is not known whether they are expressed as proteins (3). LAIR1b, which is the major alternate transcript, lacks most of the ECD. Of the minor transcripts, LAIR1c lacks a signal sequence, and LAIR1d and 1e lack a TM sequence. All mouse splice forms are identical in the last 90 aa of the cytoplasmic domain. LAIR1 shows high-affinity binding of collagens that results in inhibition of degranulation in a basophilic leukemia cell line (6). Human and mouse LAIR1 ECD share only 32% aa identity but, where studied, sites of expression and activity are similar (3-6). Mouse LAIR1 ECD also shares 62%, 31% and 28% aa identity with rat, canine, and bovine orthologs, respectively.

References

  1. Meyaard, L. (2003) J. Biol. Regul. Homeost. Agents 17:330.
  2. Meyaard, L. et al. (1997) Immunity 7:283.
  3. Lebbink, R.J. et al. (2004) J. Immunol. 172:5535.
  4. Verbrugge, A. et al. (2006) J. Leukoc. Biol. 79:828.
  5. Verbrugge, A. et al. (2003) Int. Immunol. 15:1349.
  6. Lebbink, R.J. et al. (2006) J. Exp. Med. 203:1419.

Long Name

Leukocyte-associated Ig-like Receptor 1

Alternate Names

CD305, HLAIR1, LAIR-1

Entrez Gene IDs

3903 (Human); 52855 (Mouse); 574531 (Rat); 102136964 (Cynomolgus Monkey)

Gene Symbol

LAIR1

UniProt

Q8BG84

Additional LAIR1 Products

Product Documents for Recombinant Mouse LAIR1 His-tag Protein, CF

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Product Specific Notices for Recombinant Mouse LAIR1 His-tag Protein, CF

For research use only

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