Recombinant Mouse LIF Protein

R&D Systems, part of Bio-Techne | Catalog # 8878-LF

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Carrier Free
8878-LF-025/CF
8878-LF-100/CF
Show More Sizes
8878-LF-500/CF
Show Fewer Sizes
Catalog #
Availability
Size / Price
Qty
With Carrier
8878-LF-025
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

E. coli

Accession #

P09056

Conjugate

Unconjugated

Applications

Bioactivity

View all LIF Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse LIF protein
Pro25-Phe203

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro25

Predicted Molecular Mass

20 kDa

SDS-PAGE

20 kDa, reducing conditions

Activity

Measured by its ability to induce IL-6 secretion by M1 mouse myeloid leukemia cells.
The ED50 for this effect is 0.1-0.6 ng/mL.

Scientific Data Images for Recombinant Mouse LIF Protein

Recombinant Mouse LIF Promotes the Expansion of Mouse Embryonic Stem Cells.

Recombinant Mouse LIF Promotes the Expansion of Mouse Embryonic Stem Cells.

Recombinant Mouse LIF Promotes the Expansion of Mouse Embryonic Stem Cells. D3 mouse ESCs were cultured with Recombinant Mouse LIF (10 ng/mL). After 15 days, and following multiple rounds of passaging, the ESCs were analyzed for pluripotency by staining with PE-conjugated Mouse Anti-Human/Mouse SSEA-1 PE-conjugated Monoclonal Antibody (Catalog # FAB2155P) and APC-conjugated Mouse Anti-Human/Mouse SSEA-4 APC-conjugated Monoclonal Antibody (Catalog # FAB1435A). ESCs continue to express the pluripotency marker SSEA-1 and lack expression of SSEA-4, a negative marker for mouse ESCs. Quadrants were set based on samples stained with isotype control antibodies.
Mouse Embryonic Stem Cells Cultured in Recombinant Mouse LIF Maintain their Pluripotency.

Mouse Embryonic Stem Cells Cultured in Recombinant Mouse LIF Maintain their Pluripotency.

Mouse Embryonic Stem Cells Cultured in Recombinant Mouse LIF Maintain their Pluripotency. D3 mouse ESCs were cultured in Recombinant Mouse LIF (10 ng/mL) for 15 days, including 4 passages. ESCs continue to express the mouse pluripotency markers, SSEA-1 (red) and SOX2 (green). SSEA-1 was visualized with the Human/Mouse SSEA-1 NorthernLights™ (NL)557-conjugated Antibody. SOX2 was visualized with the Goat Anti-Human SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) followed by the Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Polyclonal Antibody (Catalog # NL003). The cell nuclei were stained with DAPI (blue).
Recombinant Mouse LIF Stimulates IL-6 Secretion.

Recombinant Mouse LIF Stimulates IL-6 Secretion.

Recombinant Mouse LIF Stimulates IL-6 Secretion. Recombinant Mouse LIF (Catalog # 8878-LF) induces IL-6 secretion in M1 mouse myeloid leukemia cells. The ED50 for for this effect is 0.1-0.6 ng/mL

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 8878-LF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 0.2-1 mg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 8878-LF/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 0.2-1 mg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIF

Recombinant mouse LIF (leukemia inhibitory factor) is commonly used in cell culture to maintain the pluripotency of stem cells. LIF is a widely expressed pleiotropic member of the IL-6 family of cytokines (1-3). Mature mouse LIF is expressed as a highly and variably glycosylated 32-62 kDa monomer that shares 78%, 91%, 80%, 76%, and 78% aa sequence identity with human, rat, canine, bovine, and porcine LIF, respectively (4). LIF functions through a heterodimeric receptor complex containing a ligand-binding subunit, LIF R alpha/CD118, and a signal transducing subunit, gp130 (2, 4, 5). gp130 also serves as a subunit of the receptor complexes for Oncostatin M, Cardiotrophin-1, CNTF, IL-6, IL-11, and IL-27 (2, 5). A soluble form of mouse LIF R alpha can be generated by alternative splicing (6). Depending on the cells and their context, LIF either opposes or favors differentiation (2, 7). LIF produced by the uterine endometrium supports successful implantation of the embryo, promotes proliferation and maintenance of pluripotency in embryonic stem cells, and favors proliferation of progenitor cell types such as hematopoietic stem cells (2, 5, 7). However, excess LIF blocks differentiation of embryoid bodies, indicating the importance of LIF regulation (2, 5). LIF is produced by activated CD4+ T cells and is required by the thymic epithelium to support T cell maturation (2, 3). Its expression is upregulated by neuronal injury, and it promotes motor neuron survival and oligodendrocyte myelination (2, 3, 8). It is produced by the adrenal cortex and likely enhances adrenal production of cortisol and aldosterone (9). LIF can also function as an autocrine growth factor in some pancreatic cancers, but it induces differentiation in the myeloid leukemic cell line M1 (1, 10). Tumor cell-derived LIF can also induce formation of immunosuppressive tumor-associated macrophages (11). LIF promotes endometrial remodeling and differentiation of adipocytes and cardiac smooth muscle cells (2, 3, 12). It promotes regulatory T cell and inhibits Th17 cell differentiation, thus down-regulating inflammation and contributing to immune tolerance during pregnancy and in the nervous system (2, 3, 5, 7).

References

  1. Moreau, J.F. et al. (1988) Nature 336:690.
  2. Trouillas, M. et al. (2009) Eur. Cytokine Netw. 20:51.
  3. Metcalfe, S.M. (2011) Genes Immun. 12:157.
  4. Gearing, D.P. et al. (1987) EMBO J. 6:3995.
  5. Cheng, J.G. et al. (2001) Proc. Natl. Acad. Sci. USA 98:8680.
  6. Tomida, M. et al. (1993) FEBS lett. 334:193.
  7. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
  8. Slaets, H. et al. (2010) Trends Mol. Med. 16:493.
  9. Bamberger, A.M. et al. (2000) Mol. Cell. Endocrinol. 162:145.
  10. Kamohara, H. et al. (2007) Int. J. Oncol. 30:977.
  11. Duluc, D. et al. (2007) Blood 110:4319.
  12. Zouein, F.A. et al. (2013) Eur. Cytokine Netw. 24:11.

Long Name

Leukemia Inhibitory Factor

Alternate Names

D Factor, Emfilermin, HILDA, MLPLI

Entrez Gene IDs

3976 (Human); 16878 (Mouse); 403449 (Canine)

Gene Symbol

LIF

UniProt

P09056

Additional LIF Products

Product Documents for Recombinant Mouse LIF Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 8878-LF (with carrier)
Download SDS 8878-LF/CF (carrier free)

Product Specific Notices for Recombinant Mouse LIF Protein

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.