Recombinant Mouse LILRA6/CD85b/ILT8 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9320-T4

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

NP_035220

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all LILRA6/CD85b Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse LILRA6/CD85b protein
Mouse LILRA6/CD85b/ILT8
(Gly24-Asn629)
Accession # NP_035220		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly24

Predicted Molecular Mass

95 kDa

SDS-PAGE

97-118 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) is coated at 2 µg/mL, Recombinant Mouse LILRA6/CD85b Fc Chimera binds with an ED50 = 5-30 ng/mL.

Scientific Data Images for Recombinant Mouse LILRA6/CD85b/ILT8 Fc Chimera Protein, CF

Recombinant Mouse LILRA6/CD85b/ILT8 Fc Chimera Protein Bioactivity

Recombinant Mouse LILRA6/CD85b/ILT8 Fc Chimera Protein Bioactivity

When Recombinant Human Angiopoietin-like 7 (Catalog # 914-AN) is coated at 2 µg/mL, Recombinant Mouse LILRA6/CD85b/ILT8 Fc Chimera (Catalog # 9320-T4) binds with an ED50 = 5-30 ng/mL.

Formulation, Preparation and Storage

9320-T4
Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRA6/CD85b

LILRA6, also known as ILT8, CD85b, and PIR-A3 (in mouse), is a transmembrane molecule that belongs to the LILR family of immune regulatory proteins (1, 2). LILRA family members augment immune responses, while LILRB proteins inhibit signaling events (3). The extracellular region (ECD) of mouse LILRA6/PIR-A3 contains 6 immunoglobulin-like domains in its extracellular region, although the human protein contains four Ig-like domains. Within common regions of their ECDs, human LILRA6 shares 53% amino acid sequence identity with mouse PIR-A3. It contains a positively charged arginine residue in the transmembrane segment which mediates association with the signaling protein Fc epsilon RI gamma (4). LILRA6 is primarily expressed on monocytes (5). Its gene copy number is highly variable and is positively associated with the development of atopic dermatitis (2, 6). Allelic polymorphisms are differentially able to mediate immune cell detection of necrotic cells (7, 8). R&D Systems in-house testing indicates that LILRA6 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (9).

References

  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Hirayasu, K. and H. Arase (2015) J. Hum. Genet. 60:703.
  3. Anderson, K.J. and R.L. Allen (2009) Immunology 127:8.
  4. Taylor, L.S. and D.W. McVicar (1999) 94:1790.
  5. Bashirova, A.A. et al. (2014) Immunogenetics 66:1.
  6. Lopez-Alvarez, M.R. et al. (2016) Immunogenetics 68:743.
  7. Jones, D.C. et al. (2016) Oncotarget 7:15618.
  8. Lopez-Alvarez, M.R. et al. (2014) Immunogenetics 66:73.
  9. Zheng, J. et al. (2012) Nature 485:656.

Long Name

Leukocyte Immunoglobulin-like Receptor A6

Alternate Names

7M1, CD85b, ILT8, Pira3

Entrez Gene IDs

79168 (Human); 18726 (Mouse)

Gene Symbol

LILRA6

UniProt

NP_035220

Additional LILRA6/CD85b Products

Product Documents for Recombinant Mouse LILRA6/CD85b/ILT8 Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse LILRA6/CD85b/ILT8 Fc Chimera Protein, CF

For research use only

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