Recombinant Mouse Limitin/IFN-zeta Protein
R&D Systems, part of Bio-Techne | Catalog # 1535-LM
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Limitin/IFN-zeta protein
Leu22-Arg182, with a C-terminal Ile and 6-His tag
Leu22-Arg182, with a C-terminal Ile and 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Leu22
Predicted Molecular Mass
19 kDa
SDS-PAGE
25 kDa, reducing conditions
Activity
Measured in an anti-viral assay using L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681.
The ED50 for this effect is 3-15 pg/mL.
The ED50 for this effect is 3-15 pg/mL.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 1535-LM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 1535-LM/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Limitin/IFN-zeta
Limitin is a secreted interferon (IFN)-like glycoprotein. It has antiviral activity and selectively inhibits B-lineage lymphoid-cell development both in vivo and in vitro, while having little effect on myelopoiesis or erythropoiesis. Mouse limitin cDNA encodes a 182 aa residue precursor protein with an N-terminal 21 aa residue putative signal peptide. Limitin binds to the IFN-alpha / beta receptors and induces IFN regulatory factor-1. Mouse limitin displays 32%, 26% and 30% amino acid identity with mouse IFN-alpha, mouse IFN-beta, and human IFN-omega, respectively (1 - 2).
References
- Oritani, K. et al. (2001) J. Mol. Med. 79:168.
- Oritani, K. et al. (2000) Nature Med. 6:659.
Long Name
Interferon zeta
Alternate Names
IFN-z, IFNzeta
Entrez Gene IDs
319146 (Mouse)
Gene Symbol
IFNZ
UniProt
Additional Limitin/IFN-zeta Products
Product Documents for Recombinant Mouse Limitin/IFN-zeta Protein
Product Specific Notices for Recombinant Mouse Limitin/IFN-zeta Protein
For research use only
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