Recombinant Mouse LRP-6 N-Terminal Fragment Fc Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9950-LR

R&D Systems, part of Bio-Techne
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Key Product Details

Source

CHO

Accession #

NP_032540

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all LRP-6 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse LRP-6 protein
Mouse LRP-6
(Ala20-Ile628)
Accession # NP_032540		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala20

Predicted Molecular Mass

96 kDa

SDS-PAGE

100-120 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
In a Goat Anti-Mouse IgG Fc Antibody (Catalog # G-202-C) coated plate, Recombinant Mouse LRP‑6 N-Terminal Fragment Fc Chimera binds Recombinant Mouse Dkk-1 (Catalog # 5897-DK) with an ED50 of 15-120 ng/mL.

Scientific Data Images for Recombinant Mouse LRP-6 N-Terminal Fragment Fc Protein, CF

Recombinant Mouse LRP-6 N-Terminal Fragment Fc Protein Binding Activity

Recombinant Mouse LRP-6 N-Terminal Fragment Fc Protein Binding Activity

In a Goat Anti-Mouse IgG Fc Antibody (Catalog # G-202-C) coated plate, Recombinant Mouse LRP-6 N-Terminal Fragment Fc Chimera (Catalog # 9950-LR) binds Recombinant Mouse Dkk-1 (Catalog # 5897-DK) with an ED50 of 15-120 ng/mL.
Recombinant Mouse LRP-6 N-Terminal Fragment Fc Protein SDS-PAGE

Recombinant Mouse LRP-6 N-Terminal Fragment Fc Protein SDS-PAGE

2 μg/lane of Recombinant Mouse LRP‑6 N-Terminal Fragment Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 100-120 kDa and 200-220 kDa, respectively.

Formulation, Preparation and Storage

9950-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: LRP-6

The low-density lipoprotein (LDL) receptor-related protein 6 (LRP-6), along with LRP-5, constitute a distinct subgroup of the LDL receptor family (1). Both LRP-5 and LRP-6 are type I transmembrane proteins that function as Wnt co-receptors with Frizzled proteins and mediate the down-regulation of GSK-3 activity and the initiation of the canonical Wnt/beta-catenin signaling cascade (1, 2). Mouse LRP-6 consists of a 19 amino acid (aa) signal sequence, a 1351 aa extracellular domain (ECD), a 23 aa transmembrane domain, and a 20 aa cytoplasmic domain (3). The LRP-6 ECD is composed of four distinct YWTD beta-propeller (BP) motifs followed by three LDLR type A repeats (4). The first two LRP-6 BP motifs bind Wnt-1, -2, -2b, -6, -8a, -9a, -9b, and -10b, while the third and fourth BP motifs bind Wnt-3a (5-10). This product includes the first and second BP motifs of mouse LRP-6 (aa 20-628) that share 98% aa sequence identity with the first two BP motifs of rat and human LRP-6.  Dkk-1, a known antagonist of Wnt signaling, interacts with LRP-6 as a bipartite inhibitor, binding the first and third BP motifs of LRP-6 simultaneously (8, 11). Mice lacking LRP-6 die at birth with a truncation of the axial skeleton, limb defects, microphthalmia, and malformation of the urogenital system (2). Additionally, LRP-6 may be essential for osteoblast differentiation during bone remodeling and, along with LRP-5, is required for postnatal bone development (12, 13).

References

  1. Joiner, D.M. et al. (2013) Trends Endocrinol. Metab. 24:31.
  2. Pinson, K.I. et al. (2000) Nature 407:535.
  3. Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248:879.
  4. He, X. et al. (2004) Development 131:1663.
  5. Ai, M. et al. (2005) Mol. Cell. Biol. 25:4946.
  6. Gong, Y. et al. (2010) PLoS One 5:e12682.
  7. Itasaki, N. et al. (2003) Development 130:4295.
  8. Mao, B. et al. (2001) Nature 411:321.
  9. Zhang, Y. et al. (2004) Mol. Cell. Biol. 24:4677.
  10. Bourhis, E. et al. (2010) J. Biol. Chem. 285:9172.
  11. Ahn, V.E. et al. (2011) Dev. Cell 21:862.
  12. Li, C. et al. (2013) J. Bone Miner. Res. 28:2094.
  13. Riddle, R.C. et al. (2013) PLoS One 8:e63323.

Long Name

LDL Receptor-related Protein 6

Alternate Names

ADCAD2, FLJ90062, FLJ90421, LRP6

Entrez Gene IDs

4040 (Human); 16974 (Mouse)

Gene Symbol

LRP6

UniProt

NP_032540

Additional LRP-6 Products

Product Documents for Recombinant Mouse LRP-6 N-Terminal Fragment Fc Protein, CF

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Product Specific Notices for Recombinant Mouse LRP-6 N-Terminal Fragment Fc Protein, CF

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