Recombinant Mouse LRRTM3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6358-LR

R&D Systems, part of Bio-Techne
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6358-LR-050
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Key Product Details

Source

NS0

Accession #

Q8BZ81

Conjugate

Unconjugated

Applications

Bioactivity

View all LRRTM3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse LRRTM3 protein
Met1-His419, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu31

Predicted Molecular Mass

45.3 kDa

SDS-PAGE

55-65 kDa, reducing conditons

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Able to significantly enhance neurite outgrowth when immobilized as a 3 μL droplet containing 60-250 ng on a nitrocellulose coated microplate.

Formulation, Preparation and Storage

6358-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRTM3

Leucine-rich repeat transmembrane neuronal 3 (LRRTM3) is a 68 kDa type I transmembrane protein that belongs to the LRRTM family, LRR superfamily of proteins (1). Mouse LRRTM3 is 582 amino acids (aa) in length with a 30 aa signal sequence, a 390 aa extracellular region, a 21 aa transmembrane region, and a 141 aa cytoplasmic domain. Within the extracellular region, there are ten LRRs flanked by cysteine-rich domains and one potential site for N‑linked glycosylation (1, 2). The cytoplasmic region contains several tyrosine, serine, and threonine residues that have potential to be phosphorylated and thus to be involved in signal transduction (1). There is also a conserved ECEV motif that interacts with PDZ proteins (1, 3). One isoform variant exists that shows an Ile514Val transition coupled to a deletion of aa 515 ‑ 582. Mature mouse LRRTM3 shares 98% aa sequence identity with mature human LRRTM3. All LRRTM proteins are expressed in the brain with varying profiles in other tissues (3). Strong LRRTM3 expression is present prenatally at 8.5 dpc in the neural progenitors of the anterior neural plate that will form the rostral neural tube (3). Expression is also seen in the region of the presomitic mesoderm/somite boundary plus the forebrain and developing eye (3). In the adult, LRRTM3 may be expressed both on neurons and placental trophoblasts. In neurons, it has been demonstrated to positively influenced by STOX1 and subsequently to induce BACE1 cleavage of APP, thus generating A betaPP beta production and a C99 fragment (2, 4).

References

  1. Lauren, J. et al. (2003) Genomics 81:411.
  2. Majercak, J. et al. (2006) Proc. Natl. Acad. Sci. USA 103:17967.
  3. Haines, B.P. and P.W.J. Rigby (2007) Gene Expr. Patterns 7:23.
  4. van Dijk, M. et al. (2010) J. Alzheimers Dis. 19:673.

Long Name

Leucine Rich Repeat Transmembrane Neuronal 3

Alternate Names

leucine rich repeat transmembrane neuronal 3, leucine-rich repeat transmembrane neuronal protein 3, MGC131810

Entrez Gene IDs

347731 (Human); 216028 (Mouse); 294380 (Rat)

Gene Symbol

LRRTM3

UniProt

Q8BZ81

Additional LRRTM3 Products

Product Documents for Recombinant Mouse LRRTM3 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse LRRTM3 Protein, CF

For research use only

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