Recombinant Mouse MCAM/CD146 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9809-MA

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

Q8R2Y2-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all MCAM/CD146 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse MCAM/CD146 protein
Mouse MCAM
(Val24-Val563)
Accession # Q8R2Y2-1		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val24

Predicted Molecular Mass

87 kDa

SDS-PAGE

112-129 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse MCAM/CD146 Fc Chimera is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Mouse Galectin-3 (Catalog # 9039-GA) that produces 50% of the optimal binding response is 0.075-0.375 μg/mL.

Scientific Data Images for Recombinant Mouse MCAM/CD146 Fc Chimera Protein, CF

Recombinant Mouse MCAM/CD146 Fc Chimera Protein Binding Activity

Recombinant Mouse MCAM/CD146 Fc Chimera Protein Binding Activity

When Recombinant Mouse MCAM/CD146 Fc Chimera (Catalog # 9809-MA) is coated at 1 µg/mL (100 µg/mL), Recombinant Mouse Galectin-3 (Catalog # 9039-GA) binds with an ED50 of 0.075-0.375 µg/mL.

Formulation, Preparation and Storage

9809-MA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MCAM/CD146

Melanoma cell adhesion molecule (MCAM), also known as MUC18 or CD146, is a member of the immunoglobulin superfamily (IgSF), showing close sequence similarity to neural cell adhesion molecules (1). MCAM is a type I transmembrane glycoprotein that consists of a 23 aa signal peptide, a 540 aa extracellular domain (ECD), a 21 aa transmembrane segment and 64 aa cytoplasmic domain. Within the ECD, mouse MCAM shares 74% and 90% amino acid sequence identity with human and rat MCAM, respectively. Expression of MCAM has been detected in endothelial cells throughout the body and it has been shown to be involved in multiple cellular events including adhesion, migration, proliferation and differentiation (2, 3). Additionally, MCAM has been implicated in recruitment of activated T cells to inflammatory sites and is up-regulated in various inflammatory diseases (3, 4). Inhibiting MCAM signaling has been suggested as a potential therapy for diverse diseases including inflammatory bowel disease and ovarian cancer (5, 6). As a cellular adhesion molecule (CAM), MCAM functions as a molecular mediator to facilitate inter-cellular interactions of homotypic or heterotypic cells, or to intervene in interactions of cell-to-extracellular matrix for responding to physiological signal (7). MCAM has also been shown to be the functional ligand for Galectin-3 on endothelial cell surfaces (7).

References

  1. Lehmann, J.M. et al. (1989) Proc. Natl. Acad. Sci. U.S.A. 86:9891.
  2. Schrage, A. et al. (2008) Histochem Cell Biol. 129:441.
  3. Wang, Z. and Yan X. (2013) Cancer Lett. 330:150.
  4. Bardin, N. et al. (2006) Inflamm Bowel Dis. 12:16.
  5. Xing, S. et al. (2014) Am J Pathol. 184:1604.
  6. Ma, X. et al. (2017) Oncol Lett. 13:1681.
  7. Colomb, F. et al. (2017) J Biol Chem. 292:8381.

Long Name

Melanoma Cell Adhesion Molecule

Alternate Names

CD146, L-Gicerin, MUC18

Entrez Gene IDs

4162 (Human); 84004 (Mouse); 78967 (Rat)

Gene Symbol

MCAM

UniProt

Q8R2Y2-1

Additional MCAM/CD146 Products

Product Documents for Recombinant Mouse MCAM/CD146 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse MCAM/CD146 Fc Chimera Protein, CF

For research use only

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