Recombinant Mouse MDL-1/CLEC5A Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8438-CL

R&D Systems, part of Bio-Techne
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8438-CL-050
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Key Product Details

Source

NS0

Accession #

Q9R007

Conjugate

Unconjugated

Applications

Bioactivity

View all MDL-1/CLEC5A Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse MDL-1/CLEC5A protein
Tyr26-Lys190, with an N-terminal 9-His tag

Purity

>90%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

20 kDa

SDS-PAGE

34-45 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Galectin-9 is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Mouse MDL-1/CLEC5A that produces 50% optimal binding response is approximately 0.1-0.5 μg/mL.

Formulation, Preparation and Storage

8438-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MDL-1/CLEC5A

MDL-1 (Myeloid DAP12-associating lectin 1), also known as CLEC5A, is an approximately 40 kDa member of the C-type lectin family (1). Mature mouse MDL-1 is a glycosylated type 2 transmembrane protein that associates into a homodimer on the cell surface (2). It consists of a 165 amino acid (aa) extracellular domain (ECD) with one C-type lectin (CTL) domain and a juxtamembrane stalk region, a 21 aa transmembrane segment, and a 4 aa cytoplasmic domain (3). Within the ECD, mouse MDL-1 shares 67% and 81% aa sequence identity with human and rat MDL-1, respectively. The transmembrane segment contains a lysine residue that mediates interactions with the signaling protein DAP12 (3). Alternative splicing generates a short isoform of mouse MDL-1 that lacks 25 aa of the stalk region (3, 4). MDL-1 is expressed on monocytes, macrophages, and neutrophils (3, 4). Its expression is up-regulated on monocytes in rheumatoid arthritis (5). MDL-1 functions as a cell attachment receptor for all four serotypes of dengue virus as well as Japanese encephalitis virus, although the short isoform binds significantly more weakly (2, 6, 7). These interactions trigger DAP12 phosphorylation, the production of multiple inflammatory cytokines, vascular leakage, and disruption of the blood-brain barrier (6-8). This recombinant protein corresponds to the long isoform of mouse MDL-1.

References

  1. Hoving, J.C. et al. (2014) Cell. Microbiol. 16:185.
  2. Watson, A.A. et al. (2011) J. Biol. Chem. 286:24208.
  3. Bakker, A.B.H. et al. (1999) Proc. Natl. Acad. Sci. USA 96:9792.
  4. Aoki, N. et al. (2009) J. Leukoc. Biol. 85:508.
  5. Chen, D.-Y. et al. (2014) PLoS ONE 9:e86105.
  6. Chen, S.-T. et al. (2012) PLoS Pathogens 8:e1002655.
  7. Chen, S.-T. et al. (2008) Nature 453:672.
  8. Wu, M.-F. et al. (2013) Blood 121:95.

Long Name

Myeloid DAP12-associating Lectin

Alternate Names

CLEC5A, CLECSF5, MDL1

Entrez Gene IDs

23601 (Human); 23845 (Mouse)

Gene Symbol

CLEC5A

UniProt

Q9R007

Additional MDL-1/CLEC5A Products

Product Documents for Recombinant Mouse MDL-1/CLEC5A Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse MDL-1/CLEC5A Protein, CF

For research use only

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