Recombinant Mouse Meteorin-like/METRNL Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6679-MN

R&D Systems, part of Bio-Techne
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6679-MN-025
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Key Product Details

Source

CHO

Accession #

Q8VE43

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Bioactivity

View all Meteorin-like/METRNL Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Meteorin-like/METRNL protein
Gln46-Glu311

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln46 predicted

Predicted Molecular Mass

29.4 kDa

SDS-PAGE

33-37 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
This protein is able to significantly enhance neurite outgrowth in the range of 60 ng/3 μL drop-300 ng/3 μL drop.

Formulation, Preparation and Storage

6679-MN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Meteorin-like/METRNL

Meteorin-like protein (METRNL) is a 311 amino acid (aa) protein that shows 45% homology with Meteorin (in mouse). Meteorin itself is a secreted 30 kDa protein that is expressed in glial and neuronal cells and their precursors during development and in the adult (1 ‑ 3). In the adult Meteorin is primarily expressed by astrocytes (3). Meteorin promotes neurite outgrowth, glial cell differentiation, and neuronal survival following excitotoxic injury (2 ‑ 5). It also inhibits sprouting angiogenesis and promotes blood vessel maturation (1). Mouse Meteorin‑like protein is synthesized with a 45 amino acid (aa) signal peptide and is selectively expressed in the otic vesicle during inner ear development (6, 7). Mature mouse Meteorin‑like protein shares 79% and 98% aa sequence identity with human and rat Meteorin‑like protein, respectively. Alternate splicing generates an additional isoform that lacks the N‑terminal 82 amino acids including the signal peptide. R&D Systems in‑house testing indicates that Meteorin‑like protein enhances the outgrowth of neurites from cultured mouse cortical neurons. The promoter region of the METRNL gene contains a recognition site for the Pax2, Pax5, and Pax8 transcription factors, and Pax2 binds specifically to this region (7). METRNL is the only gene found within the terminus of the human chromosome 17 q‑arm which can be deleted in the rare Ring 17 syndrome (8).

References

  1. Park, J.A. et al. (2008) Glia 56:247.
  2. Jorgensen, J.R. et al. (2009) J. Mol. Neurosci. 39:104.
  3. Lee, H.S. et al. (2010) J. Cell Sci. 123:1959.
  4. Nishino, J. et al. (2004) EMBO J. 23:1998.
  5. Jorgensen, J.R. et al. (2011) Neurobiol. Dis. 41:160.
  6. Accession # Q8VE43.
  7. Ramialison, M. et al. (2008) Genome Biol. 9:R145.
  8. Surace, C. et al. (2009) Clin. Genet. 76:256.

Alternate Names

Meteorin like, METRNL

Entrez Gene IDs

284207 (Human); 210029 (Mouse); 316842 (Rat)

Gene Symbol

METRNL

UniProt

Q8VE43

Additional Meteorin-like/METRNL Products

Product Documents for Recombinant Mouse Meteorin-like/METRNL Protein, CF

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Product Specific Notices for Recombinant Mouse Meteorin-like/METRNL Protein, CF

For research use only

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