Recombinant Mouse MFG-E8 Protein

R&D Systems, part of Bio-Techne | Catalog # 2805-MF

R&D Systems, part of Bio-Techne
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Carrier Free
2805-MF-050/CF
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With Carrier
2805-MF-050
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Key Product Details

Source

NS0

Accession #

P21956

Conjugate

Unconjugated

Applications

Binding Activity

View all MFG-E8 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse MFG-E8 protein
Ala23-Cys463, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala23

Predicted Molecular Mass

49.8 kDa

SDS-PAGE

64-72 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells.
The ED50 for this effect is 10-50 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 2805-MF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 2805-MF/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MFG-E8

Milk Fat Globulin Protein E8 (MFG-E8), also known as Lactadherin, MP47, breast epithelial antigen BA46, and SED1, is a 66‑75 kDa pleiotropic secreted glycoprotein that promotes mammary gland morphogenesis, angiogenesis, and tumor progression. MFG-E8 also plays an important role in tissue homeostasis and the prevention of inflammation (1). Mouse MGF-E8 contains two N-terminal EGF-like domains, a Pro/Thr-rich segment, and two C‑terminal F5/8-type discoidin-like domains (2). It MFG-E8 shares 63% and 94% aa sequence identity with comparable regions of human and rat MFG-E8, respectively. Alternative splicing of mouse MFG-E8 generates a short isoform lacking the Pro/Thr-rich region which contains sites for O-linked glycosylation and tyrosine sulfation (3). MFG-E8 is released into the milk in complex with lipid-containing milk fat globules. It is also found in multiple other cell types including endothelial cells and smooth muscle cells of the vasculature, immature dendritic cells, at the acrosomal cap of testicular and epididymal sperm, and in epithelial cells of the endometrium (1). MFG-E8 binds to the Integrins alphaV beta3 and alphaV beta5 and potentiates the angiogenic action of VEGF through VEGF R2 (4, 5). It reduces inflammation and tissue damage in a variety of settings. MFG-E8 functions as a bridge between phosphatidylserine on apoptotic cells and Integrin alphaV beta3 on phagocytes, leading to the clearance of apoptotic debris (6). It mediates the engulfment of apoptotic bodies in atherosclerotic plaques and prion-infected brain (7, 8) and of apoptotic B cells during germinal center reactions (9, 10). MFG-E8 also promotes the removal of excess Collagen in fibrotic lungs and the regeneration of damaged intestinal epithelia (11, 12). Its tissue‑protective role impairs anti‑tumor immunity and chemotherapy‑induced apoptosis (13). MFG-E8 in the breastmilk blocks rotavirus infection in nursing babies (14).

References

  1. Raymond, A. et al. (2009) J. Cell. Biochem. 106:957.
  2. Stubbs, J.D. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8417.
  3. Hoffhines, A.J. et al. (2008) J. Biol. Chem. 284:3096.
  4. Silvestre, J.-S. et al. (2005) Nat. Med. 11:499.
  5. Borges, E. et al. (2000) J. Biol. Chem. 275:39867.
  6. Hanayama, R. et al. (2002) Nature 417:182.
  7. Ait-Oufella, H. et al. (2007) Circulation 115:2168.
  8. Kranich, J. et al. (2010) J. Exp. Med. 207:2271.
  9. Hanayama, R. et al. (2004) Science 304:1147.
  10. Kranich, J. et al. (2010) J. Exp. Med. 205:1293.
  11. Atabai, K. et al. (2009) J. Clin. Invest. 119:3713.
  12. Bu, H.-F. et al. (2007) J. Clin. Invest. 117:3673.
  13. Jinushi, M. et al. (2009) J. Exp. Med. 206:1317.
  14. Kvistgaard, A.S. et al. (2004) J. Dairy Sci. 87:4088.

Long Name

Milk Fat Globule EGF Factor 8

Alternate Names

BA46, Breast epithelial antigen BA46, Lactahedrin, Medin, MFGE8, SED1

Entrez Gene IDs

4240 (Human); 17304 (Mouse)

Gene Symbol

MFGE8

UniProt

P21956

Additional MFG-E8 Products

Product Documents for Recombinant Mouse MFG-E8 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 2805-MF (with carrier)
Download SDS 2805-MF/CF (carrier free)

Product Specific Notices for Recombinant Mouse MFG-E8 Protein

For research use only

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