Recombinant Mouse MSP/MST1 Protein

R&D Systems, part of Bio-Techne | Catalog # 6244-MS

R&D Systems, part of Bio-Techne
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Carrier Free
6244-MS-025/CF
Catalog #
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With Carrier
6244-MS-025
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Key Product Details

Source

CHO

Accession #

NP_032269

Structure / Form

Disulfide-linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all MSP/MST1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse MSP/MST1 protein
Met1-Glu716 (Cys677Ala)

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained for alpha‑chain, sequencing might be blocked: Gln19 predicted ( alpha‑chain) & Val489 ( beta‑chain)

Predicted Molecular Mass

53.7 kDa ( alpha‑chain) & 25 kDa ( beta‑chain)

SDS-PAGE

61-62 kDa & 30-31 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Mouse MSP R/Ron Fc Chimera (Catalog # 431-MS) is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse MSP/MST1 that produces 50% of the optimal binding response is found to be approximately 1 ‑ 6 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 6244-MS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 6244-MS/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSP/MST1

Macrophage stimulating protein (MSP), also known as HGF‑like protein, and scatter factor‑2, is a member of the HGF family of growth factors (1). MSP is secreted as an inactive single chain precursor (pro‑MSP) that contains a PAN/APPLE‑like domain, four kringle domains, and a peptidase S1 domain which lacks enzymatic activity (2). Mouse MSP shares 79% and 93% aa sequence identity with human and rat MSP, respectively and 42% aa sequence identity with mouse HGF. Pro‑MSP is secreted by hepatocytes under the positive and negative control of CBP in complex with either HNF‑4 or RAR, respectively (3). Circulating pro‑MSP is proteolytically cleaved in response to tissue injury to yield biologically active disulfide linked heterodimers consisting of a 45 ‑ 62 kDa alpha and a 25 ‑ 35 kDa beta chain (4, 5). Pro‑MSP can be activated by MT‑SP1, a transmembrane protease that is expressed on macrophages and is upregulated in many cancers (6). Heterodimeric MSP, as well as the isolated beta chain, binds to MSP R/Ron with high‑affinity, although only heterodimeric MSP can induce receptor dimerization and signaling (7, 8). MSP induces macrophage and keratinocyte proliferation and osteoclast activation (9, 10). It also inhibits LPS‑ or IFN‑induced iNOS and IL‑12 expression by macrophages and prevents apoptosis of epithelial cells separated from the ECM (11, 12). The substitution of cysteine 672 (in the beta chain) with alanine significantly increases the bioactivity of recombinant human MSP, apparently by limiting incorrect disulfide bond formation between the alpha and beta chains (13).

References

  1. Wang, M.-H. et al. (2002) Scand. J. Immunol.56:545.
  2. Degen, S.J. et al. (1991) Biochemistry 30:9781.
  3. Muraoka, R.S. et al. (1999) Endocrinology 140:187.
  4. Wang, M.-H. et al. (1996) J. Clin. Invest. 97:720.
  5. Nanney, L.B. et al. (1998) J. Invest. Dermatol. 111:573.
  6. Bhatt, A.S. et al. (2007) Proc. Natl. Acad. Sci. 104:5771.
  7. Wang, M.-H. et al. (1997) J. Biol. Chem. 272:16999.
  8. Danilkovitch, A. et al. (1999) J. Biol. Chem. 274:29937.
  9. Wang, M.-H. et al. (1996) Exp. Cell Res. 226:39.
  10. Kurihara, N. et al. (1998) Exp. Hematol. 26:1080.
  11. Morrison, A.C. et al. (2004) J. Immunol. 172:1825.
  12. Liu, Q.P. et al. (1999) J. Immunol. 163:6606.
  13. Wahl, R.C. et al. (1997) J. Biol. Chem. 272:15053.

Long Name

Macrophage Stimulating Protein/Macrophage Stimulating 1 [Hepatocyte Growth Factor-like]

Alternate Names

HGFL, MST1, SF2

Entrez Gene IDs

4485 (Human); 15235 (Mouse); 24566 (Rat)

Gene Symbol

MST1

UniProt

NP_032269

Additional MSP/MST1 Products

Product Documents for Recombinant Mouse MSP/MST1 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 6244-MS (with carrier)
Download SDS 6244-MS/CF (carrier free)

Product Specific Notices for Recombinant Mouse MSP/MST1 Protein

For research use only

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