Recombinant Mouse Neuroplastin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7818-NP

R&D Systems, part of Bio-Techne
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7818-NP-050
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Key Product Details

Source

NS0

Accession #

P97300

Conjugate

Unconjugated

Applications

Bioactivity

View all Neuroplastin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Neuroplastin protein
Gln29-Pro338, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln29 predicted

Predicted Molecular Mass

36 kDa

SDS-PAGE

40-65 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E18-E20 rat embryonic cortical neurons.
Recombinant Mouse Neuroplastin immobilized at 5-10 μg/mL is able to significantly induce neurite outgrowth.

Formulation, Preparation and Storage

7818-NP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuroplastin

Neuroplastin (Np65), also known as gp65 and SDR1, is a widely expressed and variably glycosylated transmembrane member of the CD147/Basigin protein family (1, 2). The 62-75 kDa mature mouse Neuroplastin consists of a 310 amino acid (aa) extracellular region (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 38 aa cytoplasmic domain (3, 4). Within the ECD, mouse Neuroplastin shares 94% and 98% aa sequence identity with human and rat Neuroplastin, respectively. Alternative splicing of mouse Neuroplastin generates additional isoforms that lack the first Ig-like domain, most of the first two Ig-like domains, and/or carry a 9 aa substitution in the cytoplasmic domain. The isoform lacking the first Ig-like domain is known as Neuroplastin-55. Neuroplastin is expressed on cerebellar Purkinje cells and granule cells, hippocampal pyramidal neurons, and retinal photoreceptors (5 - 9). It serves as a homophilic cell adhesion molecule on neurons and promotes neurite outgrowth (7, 8). The neurite outgrowth function requires its binding to and activation of FGFR1 (7, 10). In the synapse, Neuroplastin binds to multiple subunits of the GABA-A receptor, reduces synaptic expression of GluR1, and contributes to synaptic plasticity (7, 8, 11, 12). Neuroplastin overexpression can enhance tumor growth, VEGF production, and vascularization (13).

References

  1. Owczarek, S. and V. Berezin (2012) Int. J. Biochem. Cell Biol. 44:1.
  2. Langnaese, K. et al. (1998) FEBS Lett. 429:284.
  3. Shirozu, M. et al. (1996) Genomics 37:273.
  4. Langnaese, K. et al. (1997) J. Biol. Chem. 272:821.
  5. Marzban, H. et al. (2003) J. Comp. Neurol. 462:286.
  6. Bernstein, H-G. et al. (2007) Brain Res. 1134:107.
  7. Owczarek, S. et al. (2011) J. Neurochem. 117:984.
  8. Smalla, K-H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:4327.
  9. Kreutz, M.R. et al. (2001) Invest. Ophthalmol. Vis. Sci. 42:1907.
  10. Owczarek, S. et al. (2010) FASEB J. 24:1139.
  11. Sarto-Jackson, I. et al. (2012) J. Biol. Chem. 287:14201.
  12. Empson, R.M. et al. (2006) J. Neurochem. 99:850.
  13. Rodriguez-Pinto, D. et al. (2009) Cancer Immunol. Immunother. 58:221.

Alternate Names

AW554172, GP55, GP66, NPTN, SDFR1, SDR1

Entrez Gene IDs

27020 (Human); 20320 (Mouse); 56064 (Rat)

Gene Symbol

NPTN

UniProt

P97300 (Mouse)

Additional Neuroplastin Products

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Product Specific Notices for Recombinant Mouse Neuroplastin Protein, CF

For research use only

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