Recombinant Mouse Neutrophil Elastase/ELA2 Protein, CF Best Seller
R&D Systems, part of Bio-Techne | Catalog # 4517-SE
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Neutrophil Elastase/ELA2 protein
Ser27-Arg260, with a C-terminal 10-His tag
Ser27-Arg260, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser27
Predicted Molecular Mass
27 kDa
SDS-PAGE
34 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, MeOSuc-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin (MeOSuc-AAPV-AMC).
The specific activity is >500 pmol/min/µg, as measured under the described conditions.
The specific activity is >500 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
4517-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Neutrophil Elastase/ELA2
Neutrophil Elastase (ELA2), also known as polymorphonuclear leukocyte elastase, is a serine protease belonging to the chymotrypsin family. Located primarily in the azurophil granules of polymorphonuclear leukocytes, ELA2 function is the degradation of many extracellular matrix macromolecules (1, 2). alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity (3). This protein may be involved in lung emphysema, cystic fibrosis, the adult respiratory distress syndrome (ARDS), rheumatoid arthritis, tumor invasion and infectious diseases (1). The purified rmELA2 does not contain the last five amino acid residues of the deduced amino acid sequence (NP_056594).
References
- Bieth, J.G. (2004) In Handbook of Proteolytic Enzymes. Barrett, A.J., Rawlings, N.D., & Woessner, J.F. eds. pp. 1517.
- Owen, C.A. et al. (1995) J. Cell Biol. 131:775.
- Wiesner, O. et al. (2005) FEBS letters 579:5305.
Alternate Names
ELA2, ELANE, Elastase-2, Leukocyte Elastase, Medullasin
Gene Symbol
ELANE
UniProt
Additional Neutrophil Elastase/ELA2 Products
Product Documents for Recombinant Mouse Neutrophil Elastase/ELA2 Protein, CF
Product Specific Notices for Recombinant Mouse Neutrophil Elastase/ELA2 Protein, CF
For research use only
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