Recombinant Mouse NgR3/NgRH2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8426-NG

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
8426-NG-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

Q8K0S5

Conjugate

Unconjugated

Applications

Bioactivity

View all NgR3/NgRH2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse NgR3/NgRH2 protein
Cys25-Ala423, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys25

Predicted Molecular Mass

46 kDa

SDS-PAGE

61-69 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human APP695 is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse NgR3/NgRH2 that produces 50% optimal binding response is 0.03-0.18 μg/mL.

Formulation, Preparation and Storage

8426-NG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NgR3/NgRH2

NgR3/NgRH2 (also known as reticulon-4 receptor-Like 1), along with the related NgR1 and NgR2, is a member of the Nogo66 receptor (NgR) family. NgR proteins are expressed primarily by neurons in the central and peripheral mammalian nervous system. NgR proteins play an essential role in mediating axon growth inhibition induced by the structurally distinct proteins Nogo, myelin associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (OMgp) (1, 2).These proteins belong to the family of myelin-associated inhibitors (MAIs). NgR proteins are composed of 8 leucine-rich repeats (LRRs) flanked by N-terminal and C-terminal leucine-rich capping domains and are attached at the cell surface by a C-terminal glycosylphosphatidylinositol (GPI) anchor (3). Because NgR proteins have no transmembrane domain, they must be associated with membrane proteins in order to transduce cell signaling (2).  Both NGF R/TNFRSF16 (p75) and TROY/TNFRSF19 have been identified as NgR1-associated components (4, 5). Mouse NgR3 shares 88% and 96% amino acid sequence identity with human and rat NgR3 respectively. NgR1 and NgR3 serve as receptors for chondroitin sulfate proteoglycans (CSPGs) (6). CSPGs and MAIs are known to stabilize neuronal structure and influence the growth and guidance of developing neurons (7). Therefore, NgR proteins are part of a multicomponent receptor system implicated in pathways that limit neuronal growth and increase the structural stability of synapses.

References

  1. GrandPre, T. et al. (2002) Nature 417:547.
  2. Wang, K.C. et al. (2002) Nature 420:74.
  3. Fournier, A.E. et al. (2001) Nature 409:341.
  4. Shao, Z. et al. (2005) Neuron 45:353.
  5. Park, J. B. et al. (2005) Neuron 45:345.
  6. Dickendesher, T.L. et al. (2012) Nat. Neurosci. 15:703.
  7. Kantor, D.B. et al. (2004) Neuron 44:961.

Long Name

Nogo-66 Receptor-related Protein 3/Nogo-66 Receptor Homolog 2/Reticulon-4 Receptor-like 1

Alternate Names

NgRH2, RTN4RL1

Entrez Gene IDs

146760 (Human); 237847 (Mouse)

Gene Symbol

RTN4RL1

UniProt

Q8K0S5

Additional NgR3/NgRH2 Products

Product Documents for Recombinant Mouse NgR3/NgRH2 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse NgR3/NgRH2 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.