Recombinant Mouse Norrin Protein

R&D Systems, part of Bio-Techne | Catalog # 3497-NR

R&D Systems, part of Bio-Techne
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Carrier Free
3497-NR-025/CF
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With Carrier
3497-NR-025
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Key Product Details

Source

E. coli

Accession #

P48744

Structure / Form

Disulfide-linked oligomer

Conjugate

Unconjugated

Applications

Bioactivity

View all Norrin Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse Norrin protein
Lys25-Ser131, with an N-terminal Met

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

12.3 kDa (monomer)

Activity

Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells expressing human Frizzled-4 and human LRP-5.
The ED50 for this effect is 40-200 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 3497-NR
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 3497-NR/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Norrin

Norrin (also called Norrie Disease Protein or NDP) is a secreted regulatory protein that remains tightly associated with the extracellular matrix (1). At least 70 mutations of human Norrin have been identified in Norrie disease (X-linked retinal dysplasia) or in a minority of X-linked familial exudative vitreoretinopathy (FEVR). Both are disorders of retinal vascularization that cause blindness (2, 3). Norrin consists of disulfide-linked homodimers that oligomerize further via disulfide bridges to form higher order oligomers containing up to ten 12 kDa units. The cysteine-rich C-terminal domain of Norrin shows homology to von Willebrand factor, several extracellular mucin proteins, and members of the TGF-beta family. Molecular modeling studies predict that Norrin assumes the cysteine-knot structure typical of the TGF-beta family (4). Although Norrin is not related to Wnt family proteins, it functions like a Wnt protein in that it binds with high affinity to the receptor Frizzled-4, requires LDL receptor-related protein (LRP) as a co-receptor, and induces activation of the canonical Wnt signaling pathway (5). Norrin and Frizzled-4 are widely expressed, but at relatively low levels, in tissues displaying vascular phenotypes. Genetic disruption of either Norrin or Frizzled-4 results in blindness and progressive deafness due to vascular abnormalties (5-7). Female Norrin-/- mice are infertile due to abnormalities in decidual vasculature (8). Mature mouse Norrin shares 97-98% aa identity with human, canine and bovine Norrin.

References

  1. Perez-Vilar, J. and R.L. Hill (1997) J. Biol. Chem. 272:33410.
  2. Berger, W. et al. (1992) Nat. Genet. 1:199.
  3. Berger, W. and H.H. Ropers, 2001, in “The Metabolic and Molecular Bases of Inherited Diseases”, C.R. Scriver, et al., eds., pp. 5977.
  4. Meitinger, T. et al. (1993) Nat. Genet. 5:376.
  5. Xu, Q. et al. (2004) Cell 116:883.
  6. Hartzer, M.K. et al. (1999) Brain Res. Bull. 49:355.
  7. Wang, Y. et al. (2001) J. Neurosci. 21:4761.
  8. Luhmann, U.F.O. et al. (2005) Genesis 42:253.

Alternate Names

NDP

Entrez Gene IDs

4693 (Human)

Gene Symbol

NDP

UniProt

P48744

Additional Norrin Products

Product Documents for Recombinant Mouse Norrin Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS 3497-NR (with carrier)
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Product Specific Notices for Recombinant Mouse Norrin Protein

For research use only

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