Recombinant Mouse Plexin C1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5375-PC

R&D Systems, part of Bio-Techne
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5375-PC-050
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Key Product Details

Source

CHO

Accession #

Q9QZC2

Conjugate

Unconjugated

Applications

Bioactivity

View all Plexin C1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Plexin C1 protein
Ala35-Thr950, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala35

Predicted Molecular Mass

101.6 kDa

SDS-PAGE

130-145 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When recombinant mouse Plexin C1 is coated at 5 μg/mL (100 μL/well), recombinant mouse Semaphorin 7A/Fc Chimera (Catalog # 1835-S3) binds with an apparent KD <5 nM.

Formulation, Preparation and Storage

5375-PC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin C1

Plexin C1, also known as Virus-encoded semaphorin protein receptor (VESPR) and CD232, is a 210 kDa type I transmembrane glycoprotein in the C subfamily of the plexin family (1, 2). Human Plexin C1 contains a 34 amino acid (aa) signal sequence, a 910 aa extracellular domain (ECD) with one Sema-domain and two cysteine-rich Met-related sequences (MRS), a 21 aa transmembrane domain and a 603 aa cytoplasmic domain that includes features common to other plexins, including a phosphothreonine site. The mouse Plexin C1 ECD shares 85%, 78% and 65% aa identity with human, bovine and equine Plexin C1, respectively. A truncated rat Plexin C1 cDNA that encodes an apparently soluble form shares 93% aa identity with mouse Plexin C1 within aa, 263-932 (3). Plexin C1 is widely expressed in neuronal and non‑neuronal fetal and adult tissues (4). In neuronal development, its role is unclear. Semaphorin-7a (Sema7a, CD108) binds Plexin C1 in vitro and the two show a similar expression pattern during rat neuronal development, but beta1 integrins rather than Plexin C1 appear to mediate Sema7a effects on axon outgrowth (5, 6). Plexin C1 does appear to play a role in partitioning of paraventricular and supraoptic neurons in the hypothalamus, as indicated by specific defects seen in mice deleted for Plexin C1 (7). In the immune system, effects of Sema7a on T cell‑mediated inflammatory responses also appear to be mediated by beta1 integrins rather than plexins (8). However, Plexin C1 may function to oppose the effect of beta1 integrins, as it does on Sema7a-mediated spreading and dendrite formation in melanocytes (9). Its expression is lost in melanoma metastasis, suggesting a role as a tumor suppressor (10). Plexin C1 is the receptor for poxvirus (A39R protein) and herpes virus (AHVsema) semaphorin homologs, and mediates activation of monocytes and inhibition of dendritic cell and neutrophil phagocytosis and migration by A39R (2, 11, 12).

References

  1. Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363.
  2. Comeau, M.R. et al. (1998) Immunity 8:473.
  3. NCBI Reference Sequence: NP_001102214.
  4. Perala, N.M. et al. (2005) Gene Expr. Patterns 5:355.
  5. Pasterkamp, R.J. et al. (2007) BMC Dev. Biol. 7:98.
  6. Pasterkamp, R.J. et al. (2003) Nature 424:398.
  7. Xu, C. & C-M. Fan (2007) Mol. Endocrinol. 21:1234.
  8. Suzuki, K. et al. (2007) Nature 446:680.
  9. Scott, G.A. et al. (2007) J. Invest. Dermatol. 128:151.
  10. Lazova, R. et al. (2009) Am. J. Dermatopathol. 31:177.
  11. Walzer, T. et al. (2005) J. Immunol. 174:51.
  12. Walzer, T. et al. (2005) Eur. J. Immunol. 35:391.

Alternate Names

CD232, PLXNC1, VESPR

Entrez Gene IDs

10154 (Human); 54712 (Mouse); 362873 (Rat)

Gene Symbol

PLXNC1

UniProt

Q9QZC2

Additional Plexin C1 Products

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