Recombinant Mouse ROR2 mFc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11425-RO

R&D Systems, part of Bio-Techne
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11425-RO-050
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Key Product Details

Source

NS0

Accession #

NP_038874.3

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all ROR2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse ROR2 protein
Mouse ROR2
(Glu34-Gly403)
Accession # NP_038874.3		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu34

Predicted Molecular Mass

68 kDa

SDS-PAGE

80-88 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Mouse ROR2 mFc Chimera (Catalog # 11425-RO) binds Human ROR2 Antibody (Catalog # AF2064) with an ED50 of 0.600-7.20 ng/mL.

Scientific Data Images for Recombinant Mouse ROR2 mFc Chimera Protein, CF

Recombinant Mouse ROR2 mFc Chimera Protein Binding Activity.

Recombinant Mouse ROR2 mFc Chimera Protein (Catalog # 11425-RO) binds Human ROR2 Antibody (AF2064) with an ED50 of 0.600-7.20 ng/mL.

Recombinant Mouse ROR2 mFc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Mouse ROR2 mFc Chimera Protein (Catalog # 11425-RO) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 80-88 kDa and 160-180 kDa, respectively.

Formulation, Preparation and Storage

11425-RO
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROR2

ROR2 (Receptor Tyrosine Kinase-like Orphan Receptor 2) is a member of the ROR family of receptor tyrosine kinases and is important for skeletal development, including bone and cartilage formation, as well as for the development of the central nervous system (1-5). Mature mouse ROR2 contains a 370 amino acid (aa) extracellular domain (ECD) and a 520 aa cytoplasmic tail containing an tyrosine kinase domain. The ROR2 ECD features an immunoglobulin domain, a cysteine-rich domain that resembles the Wnt-binding sites of Frizzled proteins, and a kringle domain. The ECD of mouse ROR2 shares 94% and 99% aa sequence identity with the ECD of human and rat ROR2, respectively. ROR2 binds the Wnt family ligand, Wnt-5a, to activate non-canonical Wnt signaling pathways (6-8). ROR2 is broadly expressed during embryonic development and can be found in cells of all three germ layers as well as in most organ tissues (9, 10). Activation of ROR2 signaling promotes cellular proliferation, differentiation, cell-polarization, and migration. ROR2 is important for osteogenic and chondrogenic differentiation of mesenchymal stem cells (11, 12). Mutations in ROR2 are associated with the skeletal disorders, brachydactyly type B and Robinow syndrome (6). ROR2 is also involved in neurite outgrowth and synapse development in the brain (4). A variety of tumors have been found to over-express ROR2. Depending upon the cellular context, ROR2 can act as a tumor suppressor or promoter (13). Expression of ROR2 within carcinomas is correlated with a poor prognosis of survival and increased rates of recurrence (14-16).

References

  1. DeChiara, T.M. et al. (2000) Nat. Genet. 24:271.
  2. Petrova, I.M. et al. (2014) Mol. Neurobiol. 49:303.
  3. Takeuchi, S. et al. (2000) Genes Cells 5:71.
  4. Paganoni, S. and A. Ferreira (2003) J. Neurosci. Res. 73:429.
  5. Endo, M. et al. (2012) J. Cell. Sci. 125:2017.
  6. Minami, Y. et al. (2010) Dev. Dyn. 239:1.
  7. Oishi, I. et al. (2003) Genes Cells 8:645.
  8. Wallkamm, V. et al. (2014) PLoS ONE 9:e109428.
  9. Al-Shawi, R. et al. (2001) Dev. Genes. Evol. 211:161.
  10. Matsuda, T. et al. (2001) Mech. Dev. 105:153.
  11. Cai, S.X. et al. (2014) J. Cell. Physiol. 229:791.
  12. Xin, H. et al. (2013) Int. J. Mol. Med. 31:583.
  13. Ford, C.E. et al. (2013) Int. J. Cancer 133:779.
  14. Rasmussen, N.R. et al. (2014) PLoS ONE 9:e116101.
  15. Mei, H. et al. (2014) Biochem. Biophys. Res. Commun. 453:703.
  16. Lu, B.J. et al. (2012) Mol. Med. Rep. 5:1033.

Long Name

Receptor Tyrosine Kinase-like Orphan Receptor 2

Alternate Names

BDB1, NTRKR2

Entrez Gene IDs

4920 (Human); 26564 (Mouse)

Gene Symbol

ROR2

UniProt

NP_038874.3

Additional ROR2 Products

Product Documents for Recombinant Mouse ROR2 mFc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse ROR2 mFc Chimera Protein, CF

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