Recombinant Mouse S100A8 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9877-S8

R&D Systems, part of Bio-Techne
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9877-S8-050
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Key Product Details

Source

E. coli

Accession #

P27005

Conjugate

Unconjugated

Applications

Bioactivity

View all S100A8 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse S100A8 protein
Met1-Glu89

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1 & Pro2

Predicted Molecular Mass

10 kDa

SDS-PAGE

7 kDa, reducing conditions

Activity

Measured by its ability to induce CXCL1/KC secretion by C3H10T1/2 mouse embryonic fibroblast cells.
The ED50 for this effect is 1.5-9 μg/mL.

Scientific Data Images for Recombinant Mouse S100A8 Protein, CF

Recombinant Mouse S100A8 Protein Bioactivity

Recombinant Mouse S100A8 Protein Bioactivity

Recombinant Mouse S100A8 (Catalog # 9877-S8) induces CXCL1/KC secretion in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 1.5-9 μg/mL.
Recombinant Mouse S100A8 Protein SDS-PAGE

Recombinant Mouse S100A8 Protein SDS-PAGE

1 μg/lane of Recombinant Mouse S100A8 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 7 kDa.

Formulation, Preparation and Storage

9877-S8
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and TCEP with Trehalose.
Reconstitution
Reconstitute at 250 μg/mL in water.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: S100A8

Mouse S100A8, also known as CP-10, Calgranulin A and MRP8, is a 10 kDa member of the S100 family of calcium-binding proteins (1, 2). Mouse S100A8 is an 89 amino acid protein that contains short sequential modules, including an N-terminal alpha -helix, a Ca++-binding EF-hand segment, a short central linker region, a second EF-hand segment, and a C-terminal alpha -helix. S100A8 forms homodimer, and also heterodimerizes with S100A9. In the presence of Ca++, the heterodimers form heterotetramers (3, 4). The dimeric S100 A8/A9 complex is found both intracellularly and extracellularly. It binds to heparan sulfate and is chemotactic for PMNs and macrophages. Like S100A9, S100A8 is up-regulated in neutrophils and monocytes at sites of inflammation (e.g. psoriasis, rheumatoid arthritis, cardiac ischemia) and are present at elevated concentrations in rheumatoid arthritis synovial fluid (5-7). It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. In addition, S100A8 was shown to activate lung alveolar epithelial cells, osteoclasts, and chondrocytes in TLR4-dependent manner, inducing proinflammatory cytokines and chemokines including, IL-6, IL-8, KC, and MCP-1 (8-10). In osteoarthritic chondrocytes, S100A8 and A9 also promoted expression of matrix metalloproteinases (MMP-1, -3, -9, and -13) potentially affecting to cartilage breakdown (10). The amino acid sequence of mouse S100A8 is 80% and 57% identical to that of rat and human S100A8, respectively (11, 12).

References

  1. Averill, M.M. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:223.
  2. Kerkhoff, C. et al. (2012) Exp. Dermatol. 21:822.
  3. Ryckman, C. et al. (2003) J. Immunol. 170:3233.
  4. Vogl, T. et al. (2006) Biochim. Biophys. Acta 1763:1298.
  5. Siegenthaler, G. et al. (1997) J. Biol. Chem. 272:9371.
  6. Sunahori, K. et al. (2006) Arthritis Res. Ther. 8:R69.
  7. Volz, H.C. et al. (2012) Basic Res. Cardiol. 107:250.
  8. Chakraborty D. et al. (2017) Front Immunol. 8:1493
  9. Grevers L.C. Arthritis Rheum. 63:1365.
  10. Schelbergen R.F. Arthritis Rheum. 64:1477.
  11. Lagasse, E. and I.L. Weissman (1992) Blood 79:1907.
  12. Lackmann, M. et al. (1993) J. Immunol. 150:2981.

Long Name

S100 Calcium Binding Protein A8

Alternate Names

Calgranulin A, CFAG, MRP8

Entrez Gene IDs

6279 (Human); 20201 (Mouse)

Gene Symbol

S100A8

UniProt

P27005

Additional S100A8 Products

Product Documents for Recombinant Mouse S100A8 Protein, CF

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Product Specific Notices for Recombinant Mouse S100A8 Protein, CF

For research use only

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