Recombinant Mouse SCARA5 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4754-SR
Key Product Details
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived mouse SCARA5 protein
Arg83-Pro491, with an N-terminal 6-His tag
Arg83-Pro491, with an N-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
His
Predicted Molecular Mass
45.5 kDa
SDS-PAGE
60-90 kDa, reducing conditions
Activity
Measured by its ability to bind fluorescein-conjugated S. aureus Bioparticles. Jiang, Y. et al. (2006) J. Biol. Chem. 281:11834.
The ED50 for this effect is 0.25-1.25 µg/mL.
The ED50 for this effect is 0.25-1.25 µg/mL.
Formulation, Preparation and Storage
4754-SR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: SCARA5
Scavenger receptor A5 (SCARA5; also known as testis expressed scavenger receptor Tesr) is a Class A scavenger receptor that is related to CL-P1, MARCO, SCARA3, and SR-A1. All are type II transmembrane proteins that contain a collagenous stalk (1 - 3). Mature mouse SCARA5 consists of a 60 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 410 aa C-terminal extracellular domain (ECD) that contains a coiled-coil, a collagen-like, and a scavenger receptor cysteine-rich (SRCR) region (4, 5). Within the ECD, mouse SCARA5 shares 87% and 98% aa sequence identity with human and rat SCARA5, respectively. It shares 24% - 29% aa sequence identity with CL-P1, MARCO, SCARA3, and SR-A1. Alternative splicing generates one isoform that lacks the SRCR domain and a second that additionally lacks the cytoplasmic and transmembrane regions. SCARA5 is a cell surface disulfide-linked homotrimer of > 250 kDa (5). It is highly expressed in testicular Sertoli and germ cells and more weakly in the epithelia of other tissues (4, 5). During mouse development, SCARA5 expression is attenuated in female embryos at the time of sex determination, whereas it is maintained in the developing testis (4). SCARA5 is re-expressed in the adult ovary (4). SCARA5 binds heat-killed bacterial particles but not yeast particles or modified LDL (5).
References
- Murphy, J.E. et al. (2005) Atherosclerosis 182:1.
- Mukhopadhyay, S. And S. Gordon (2004) Immunobiology 209:39.
- Sarrias, M.R. et al. (2004) Crit. Rev. Immunol. 24:1.
- Sarraj, M.A. et al. (2002) Dev. Dyn. 234:1026.
- Jiang, Y. et al. (2006) J. Biol. Chem. 281:11834.
Long Name
Scavenger Receptor Class A Member 5
Alternate Names
Tesr
Gene Symbol
SCARA5
UniProt
Additional SCARA5 Products
Product Documents for Recombinant Mouse SCARA5 Protein, CF
Product Specific Notices for Recombinant Mouse SCARA5 Protein, CF
For research use only
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