Recombinant Mouse Serglycin/SRGN His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10190-SN

R&D Systems, part of Bio-Techne
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10190-SN-050
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Key Product Details

Source

HEK293

Accession #

P13609

Conjugate

Unconjugated

Applications

Bioactivity

View all Serglycin/SRGN Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mouse Serglycin/SRGN protein
Mouse Serglycin/SRGN
(Tyr26-Ile152)
Accession # P13609		 HPGGGSGGGSGGGS HHHHHH
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr26

Predicted Molecular Mass

16 kDa

SDS-PAGE

55 kDa and above, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse CD44 Fc Chimera (Catalog # 6127-CD) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Mouse Serglycin/SRGN His-tag (Catalog # 10190-SN) binds with an ED50 of 1.5-15 ng/mL.

Scientific Data Images for Recombinant Mouse Serglycin/SRGN His-tag Protein, CF

Recombinant Mouse Serglycin/SRGN His-tag Protein Binding Activity

Recombinant Mouse Serglycin/SRGN His-tag Protein Binding Activity

When Recombinant Mouse CD44 Fc Chimera (Catalog # 6127-CD) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Mouse Serglycin/SRGN His-tag (Catalog # 10190-SN) binds with an ED50 of 1.5-15 ng/mL.
Recombinant Mouse Serglycin/SRGN His-tag Protein SDS-PAGE

Recombinant Mouse Serglycin/SRGN His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse Serglycin/SRGN His-tag (Catalog # 10190-SN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining. Due to heavily O-linked glycosylation, it runs as a smear above 55 kDa.

Formulation, Preparation and Storage

10190-SN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serglycin/SRGN

Serglycin, also known as hematopoetic proteoglycan core protein or secretory granule proteoglycan core protein, is a 16 kDa polypeptide with extensive glycosylation modification. Proteoglycans (PGs) are synthesized by all cells and distributed in all tissues. There are three main groups of PGs based on localization: the cell-surface associated PGs, such as Syndecans and Glypicans, the matrix secreted PGs, such as Versican and Perlecan, and the intracellular PGs, with Serglycin being the only known member of this subfamily to date (1). Serglycin was initially found as a hematopoietic PG present in intracellular secretory compartments. Recent studies suggest that Serglycin is expressed by a variety of cell types, where it participates in both physiological functions and pathologic conditions (2, 3). Serglycin is important in formation of secretory granules and mediates storage of various compounds in secretory vesicles. In connective tissue and mucosal mast cells, Serglycin is required for storage and secretion of chemicals and proteases such as histamine, chymase, tryptase and carboxypeptidase (4). Serglycin is also important for storage of granzyme B in T-lymphocytes and localizing neutrophil elastase in azurophil granules of neutrophils (5). It has been found that Serglycin regulates the secretion of TNF-alpha in macrophages (6). Serglycin consists of a small core protein containing serine/glycine repeats, with eight such G-S repeats found in human Serglycin and ten in mouse Serglycin (1). Each serine of this repeat region is a potential GAG attachment site. The size of the PG varies according to the GAG chain length, number, and type (7). In connective tissue mast cells the covalently attached glycosaminoglycan is heparin, whereas mucosal mast cells and activated macrophages contain oversulfated chondroitin sulfate (type E) (8). Within the core protein, mouse Serglycin shares 66% and 38% amino acid identity to rat and human Serglycin. Serglycin can inhibit both the classical and lectin pathways of complement through direct interaction with C1q and mannose-binding lectin (9). In vitro study using human umbilical vein endothelial cells (HUVECs) suggested lipopolysaccharide and interleukin-1 beta played important role in Serglycin synthesis and secretion (10). In addition, Serglycin can promote myeloma cell adhesion to bone marrow stromal cells as well as of non-small cell lung cancer cell migration, invasion and its colonization in the lung and liver through CD44 (11, 12). Serglycin is directly involved in myeloma tumor progression thus suggesting it may be a therapeutic target for multiple myeloma (11).

References

  1. Theocharis, A.D. et al. (2010) FEBS J. 277:3904.
  2. Kolset, S.O. and Pejler G. J. (2011) Immunol. 187:4927.
  3. Scully, O.J. et al. (2012) Anat Rec (Hoboken) 295: 1415.
  4. Abrink, M. et al. (2004) J. Biol. Chem. 279:40897.
  5. Grujic, M. et al. (2005) J. Biol. Chem. 280:33411.
  6. Zernichow, L. et al. (2006) J. Biol. Chem. 281:26792.
  7. Schick, B.P. (2010) Prog Mol Biol Transl Sci. 93:235.
  8. Toyama-Sorimachi, N. et al. (1997) J Biol Chem. 272:26714.
  9. Skliris, A. et al. (2011) Eur. J. Immunol. 41:437.
  10. Reine, T. et al. (2014) Biochim Biophys Acta. 1840:2498.
  11. Purushothaman, A. (2014) J. Biol. Chem. 289:5499.
  12. Guo, J. et al. (2017) Oncogene 36:2457.

Long Name

Hematopoietic Proteoglycan Core Protein

Alternate Names

SRGN

Entrez Gene IDs

5552 (Human); 19073 (Mouse)

Gene Symbol

SRGN

UniProt

P13609

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