Recombinant Mouse Serpin A1a Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7690-PI

R&D Systems, part of Bio-Techne
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7690-PI-010
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Key Product Details

Source

NS0

Accession #

AAH11040

Conjugate

Unconjugated

Applications

Inhibition Activity

View all Serpin A1a Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Serpin A1a protein
Glu25-Lys413, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu25

Predicted Molecular Mass

44 kDa

SDS-PAGE

50-60 kDa, reducing conditions

Activity

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 is <5 nM, as measured under the described conditions.

Formulation, Preparation and Storage

7690-PI
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Serpin A1a

Serpin A1a, also known as alpha-1-antitrypsin, belongs to the serpin superfamily of proteinase inhibitors. It is one of five alpha 1-proteinase inhibitors produced in mice. Serpin A1a is produced in the liver, then secreted into the bloodstream. It inhibits a number of serine proteases, including elastase, trypsin, plasmin, and thrombin (1, 2). Serpin A1a’s major function is to protect the lungs from neutrophil elastase. Serpin A1a deficiency can cause complications such as emphysema, and chronic obstructive pulmonary disease (COPD).

References

  1. Gettins, P.G. (2002) Chem. Rev. 102:4751.
  2. Kalsheker N.A. (1989) Biosci. Rep. 9:129. 3. Ritchie, R.F. et al. (2000) J. Clin. Lab. Anal. 14:284.

Alternate Names

Aat2, Dom1, PI1, Spi1-1

Entrez Gene IDs

5265 (Human); 20700 (Mouse)

Gene Symbol

SERPINA1

UniProt

AAH11040

Additional Serpin A1a Products

Product Documents for Recombinant Mouse Serpin A1a Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Serpin A1a Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

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