Recombinant Mouse Serpin A3N Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4709-PI
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Serpin A3N protein
Phe21-Lys418, with a C-terminal 6-His tag
Phe21-Lys418, with a C-terminal 6-His tag
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Phe21
Predicted Molecular Mass
46 kDa
SDS-PAGE
59 kDa, reducing conditions
Activity
Measured by its ability to inhibit Granzyme B cleavage of tert-butoxycarbonyl-Ala-Ala-Asp-thiobenzyl ester (Boc-AAD-SBzl).
The IC50 is <25 nM, as measured under the described conditions.
The IC50 is <25 nM, as measured under the described conditions.
Formulation, Preparation and Storage
4709-PI
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Serpin A3N
Serpin A3N is a serine protease inhibitor that is structurally related to alpha1-antichymotrypsin encoded by the SERPINA3 gene (1). Serpin A3N is highly expressed in brain, testis, lung, thymus, and spleen (2). Serpin A3N secreted by Sertoli cells may regulate the activity of locally produced Granzyme B (3). Granzyme B inhibition by Serpin A3N may therefore regulate Granzyme B-mediated killing by cytotoxic lymphocytes, providing a means to disable cell-mediated immune responses.
References
- Forsyth, S. et al. (2003) Genomics 81:336.
- Horvath, A. J. et al. (2004) J. Mol. Evol. 59:488.
- Hirst, C. E. et al. (2001) Mol. Hum. Reprod. 7:1133.
Alternate Names
Spi2
Gene Symbol
SERPINA3N
UniProt
Additional Serpin A3N Products
Product Documents for Recombinant Mouse Serpin A3N Protein, CF
Product Specific Notices for Recombinant Mouse Serpin A3N Protein, CF
For research use only
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